3,3',5,5'-四甲基联苯胺作为辣根过氧化物酶在酶免疫测定中的Ames试验阴性显色剂。
3,3',5,5' - Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay.
作者信息
Bos E S, van der Doelen A A, van Rooy N, Schuurs A H
出版信息
J Immunoassay. 1981;2(3-4):187-204. doi: 10.1080/15321818108056977.
The use of 3,3',5,5' - tetramethylbenzidine as non-mutagenic chromogen for the end point determination in enzyme-immunoassay (EIA) is described. In sandwich EIAs for HCG and HBsAg and in a competitive EIA for testosterone, the colour yield with TMB was superior to that obtained with o-phenylene diamine (OPD), which was by far the best chromogen for horse-radish peroxidase until now. This led to an improvement of sensitivity and precision of the assays and makes EIA even more competitive with other types of immunoassays.
本文描述了使用3,3',5,5'-四甲基联苯胺作为非诱变显色剂用于酶免疫测定（EIA）终点测定的情况。在用于人绒毛膜促性腺激素（HCG）和乙型肝炎表面抗原（HBsAg）的夹心EIA以及用于睾酮的竞争性EIA中，TMB产生的颜色比邻苯二胺（OPD）产生的颜色更好，而OPD是迄今为止辣根过氧化物酶的最佳显色剂。这提高了测定的灵敏度和精密度，使EIA在与其他类型免疫测定竞争中更具优势。
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