Kurita Y, Tsuboi R, Ueki R, Rifkin D B, Ogawa H
Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan.
Arch Dermatol Res. 1992;284(4):193-7. doi: 10.1007/BF00375792.
The immunohistochemical localization of basic fibroblast growth factor (bFGF) was examined during wound healing in mouse skin. Frozen sections taken from the rounded skin defects were reacted with polyclonal anti-human recombinant bFGF IgG followed by incubation with FITC-conjugated IgG. The basal layer keratinocytes and hair bulbs at the wound edge were strongly stained with this antibody. In the reepithelized area, several layers of keratinocytes from the basal layer were positively stained regardless of the time after wounding. These findings suggest that germinative keratinocytes which express bFGF function as leading cells in the covering of the wound defect. However, dermal granulation tissue, including capillary endothelial cells, fibroblasts and macrophages unexpectedly did not demonstrate any immunoreactivity throughout the process of wound healing. Simultaneous histochemical investigation using cultivated mouse keratinocytes and bovine aortic endothelial cells showed primarily cytoplasmic fluorescence. The discrepancy in the staining patterns of endothelial cells in vivo and in vitro suggests that immunoreactive bFGF is either not expressed in vivo, or is processed or masked.
在小鼠皮肤伤口愈合过程中，对碱性成纤维细胞生长因子（bFGF）进行了免疫组织化学定位研究。从圆形皮肤缺损处获取的冰冻切片与多克隆抗人重组bFGF IgG反应，随后与异硫氰酸荧光素（FITC）偶联的IgG孵育。伤口边缘的基底层角质形成细胞和毛球被该抗体强烈染色。在再上皮化区域，无论伤后时间如何，基底层的几层角质形成细胞均呈阳性染色。这些发现表明，表达bFGF的生发角质形成细胞在覆盖伤口缺损过程中起引导细胞的作用。然而，包括毛细血管内皮细胞、成纤维细胞和巨噬细胞在内的真皮肉芽组织在整个伤口愈合过程中意外地未显示任何免疫反应性。使用培养的小鼠角质形成细胞和牛主动脉内皮细胞进行的同步组织化学研究显示主要为细胞质荧光。体内和体外内皮细胞染色模式的差异表明，具有免疫反应性的bFGF要么在体内不表达，要么被加工或掩盖。
