Keegan T E, Heaton A, Holme S, Owens M, Nelson E J
American Red Cross, Mid-Atlantic Regional Blood Services, Norfolk, Virginia 23501.
Br J Haematol. 1992 Sep;82(1):114-21. doi: 10.1111/j.1365-2141.1992.tb04602.x.
A centrifugal method of red cell density separation was utilized for unit processing in these studies to determine the quality of the lighter fraction (neocytes) after storage for up to 42 d and to evaluate whether the heavier fraction (gerocytes) deteriorated more rapidly than neocytes during storage. Each unit was passed through a Leukotrap filter to remove white cells prior to density separation. Red cell recovery and survival were evaluated using double label technetium-99m with either chromium-51 or nonradioisotopic chromium which permitted concurrent paired analysis. In vivo neocyte red cell recovery, as tested on the same 11 donors on days 1, 7 and 42 of storage, was effectively unchanged. Recovery and survival half-life (that is, the number of days after transfusion at which half of the cells infused remain in the circulation) of 42 d stored gerocytes were significantly lower than similarly stored neocytes (75.5 +/- 7.2% and 20.1 +/- 6.5 d for gerocytes versus 84.4 +/- 4.9% and 39.0 +/- 9.0 d for neocytes). One-day stored neocytes showed a 16.5% increase in red cell availability over the combined average for 42 d stored neocytes and gerocytes. Statistically, while there were significantly higher ATP, 2,3-DPG, and lactate levels pre-storage by paired t-test for neocytes compared to gerocytes, by day 42 there were no significant differences detected between the two red cell fractions by any of the in vitro variables measured. These studies suggest that this simple separation technique for leucocyte-poor red cell units provides a neocyte population with improved viability and the potential for increased transfusion intervals in chronically transfused patients.
在这些研究中，采用离心法进行红细胞密度分离以处理单位血液，目的是确定储存长达42天的较轻部分（新生红细胞）的质量，并评估较重部分（衰老红细胞）在储存过程中是否比新生红细胞更快地恶化。在进行密度分离之前，每个单位血液都通过Leukotrap过滤器以去除白细胞。使用双标记锝-99m与铬-51或非放射性同位素铬来评估红细胞的回收率和存活率，这允许进行同时配对分析。在储存第1天、第7天和第42天对相同的11名供体进行测试，体内新生红细胞的回收率实际上没有变化。储存42天的衰老红细胞的回收率和存活半衰期（即输血后输注的细胞有一半仍留在循环中的天数）明显低于同样储存的新生红细胞（衰老红细胞分别为75.5±7.2%和20.1±6.5天，新生红细胞分别为84.4±4.9%和39.0±9.0天）。储存1天的新生红细胞与储存42天的新生红细胞和衰老红细胞的综合平均值相比，红细胞可用性增加了16.5%。统计学上，通过配对t检验，储存前新生红细胞的ATP、2,3-二磷酸甘油酸和乳酸水平显著高于衰老红细胞，但到第42天，通过所测量的任何体外变量，在这两个红细胞部分之间未检测到显著差异。这些研究表明，这种用于制备少白细胞红细胞单位的简单分离技术可提供具有更高活力的新生红细胞群体，并有可能延长长期输血患者的输血间隔。
