Picot C, Girot R, Loutounda J, Mattlinger B, Maier-Redelsperger M, Feo C, Chevalier A, Barritault L
Département d'Hématologie et de Transfusion Sanguine, Hôpital Necker-Enfants Malades, Paris, France.
Eur J Haematol. 1987 Sep;39(3):214-20. doi: 10.1111/j.1600-0609.1987.tb00760.x.
The transfusion of young red blood cells (neocytes) is a complementary approach to the present treatment of thalassaemia major patients. Fresh neocytes were harvested from 34 volunteers with the IBM 2991 cell washer (CW). The upper part of 24 units was separated into 6 aliquots (T1-T6) of 15 ml each and the residue homogenized. Four biological criteria were used to evaluate young red cell quality, the erythrocyte pyruvate kinase activity, the mean corpuscular volume, the reticulocyte count and ektacytometry; each sample (T1-T6) showed an enrichment with neocytes as compared with the standard unit. A real efficiency of this technique with the IBM 2991 CW was obtained with the first third of the unit, corresponding to the T1-T4 fractions. The estimation of the theoretical mean life span of T1-T2 was 270 days. The last 10 units were separated into two halves: the enrichment of the first half was worse than in the first third of the previous technique. We concluded that the transfusional program of thalassaemia patients could profit by the use of the most enriched part of a standard blood unit with the IBM 2991 CW.
输注年轻红细胞（新生红细胞）是目前重度地中海贫血患者治疗的一种补充方法。使用IBM 2991细胞洗涤器（CW）从34名志愿者中采集新鲜的新生红细胞。将24个单位的上部均分成6个15毫升的等分试样（T1 - T6），其余部分匀浆。采用四项生物学标准评估年轻红细胞质量，即红细胞丙酮酸激酶活性、平均红细胞体积、网织红细胞计数和红细胞变形性测定；与标准单位相比，每个样本（T1 - T6）均显示出新生红细胞富集。使用IBM 2991 CW，该技术的实际效率在单位的前三分之一（对应于T1 - T4组分）时即可获得。T1 - T2的理论平均寿命估计为270天。最后10个单位均分成两半：前半部分的富集程度不如前一种技术前三分之一的情况。我们得出结论，地中海贫血患者的输血方案可通过使用IBM 2991 CW从标准血液单位中最富集的部分获益。
