Tyeryar F J, Lawton W D, MacQuillan A M
J Bacteriol. 1968 Jun;95(6):2062-9. doi: 10.1128/jb.95.6.2062-2069.1968.
A sequential replication map of the chromosome of Bacillus licheniformis was constructed by employing the method of gene-frequency analysis presented by Yoshikawa and Sueoka. Our analysis of 11 genetic markers was based on the hypothesis that the chromosome initiated replication at a fixed origin and proceeded in a linear fashion to the terminus. The proposed locations of markers were validated by cotransformation and cotransduction analyses. Bacteriophage SP-15 cotransduced markers that failed to show linkage by transformation.
通过采用吉川和末冈提出的基因频率分析方法,构建了地衣芽孢杆菌染色体的连续复制图谱。我们对11个遗传标记的分析基于这样的假设:染色体在一个固定的起点开始复制,并以线性方式进行到终点。标记的提议位置通过共转化和共转导分析得到验证。噬菌体SP-15共转导了那些通过转化未能显示连锁关系的标记。