Argyris B F, Cotellessa A
Transplantation. 1979 Nov;28(5):372-6. doi: 10.1097/00007890-197911000-00005.
Suppressor cell activity is high in the spleen of C57BL/6 mice sensitized to the allogeneic P-815 mastocytoma. The suppressor cells inhibit proliferation of normal mouse spleen cells in mixed lymphocyte cultures. Preincubation of tumor-allosensitized spleen cells on monolayers of syngeneic or allogeneic macrophages reduces suppressor activity. Preincubation on monolayers of spleen cells does not affect suppressor activity. Suppressor cells can be retrieved from the macrophage monolayers used for adsorption. Heat-killed macrophages fail to adsorb out suppressor cell activity. Incubation of tumor-allosensitized spleen cells on syngeneic macrophage monolayers does not reduce cytotoxic cell activity. Removal of suppressor cell activity by macrophage adsorption restores helper activity of tumor-allosensitized spleen cells. These results demonstrate that suppressor cell activity can be reduced by contact with macrophages and point to an additional role for the macrophage in regulating the immune response to tumors.
在对同种异体P - 815肥大细胞瘤致敏的C57BL / 6小鼠脾脏中，抑制细胞活性很高。这些抑制细胞在混合淋巴细胞培养中抑制正常小鼠脾细胞的增殖。将肿瘤同种异体致敏的脾细胞在同基因或异基因巨噬细胞单层上预孵育可降低抑制活性。在脾细胞单层上预孵育不影响抑制活性。可从用于吸附的巨噬细胞单层中回收抑制细胞。热灭活的巨噬细胞不能吸附掉抑制细胞活性。将肿瘤同种异体致敏的脾细胞在同基因巨噬细胞单层上孵育不会降低细胞毒性细胞活性。通过巨噬细胞吸附去除抑制细胞活性可恢复肿瘤同种异体致敏脾细胞的辅助活性。这些结果表明，抑制细胞活性可通过与巨噬细胞接触而降低，并指出巨噬细胞在调节对肿瘤的免疫反应中还有额外作用。
