通过稀释法估算传染性细菌或病毒颗粒的数量
ESTIMATION OF THE NUMBER OF INFECTIOUS BACTERIAL OR VIRAL PARTICLES BY THE DILUTION METHOD.
作者信息
SELIGMAN S J, MICKEY M R
出版信息
J Bacteriol. 1964 Jul;88(1):31-6. doi: 10.1128/jb.88.1.31-36.1964.
Seligman, Stephen J. (University of California, Los Angeles), and M. Ray Mickey. Estimation of the number of infectious bacterial or viral particles by the dilution method. J. Bacteriol. 88:31-36. 1964.-For viral or bacterial systems in which discrete foci of infection are not obtainable, it is possible to obtain an estimate of the number of infectious particles by use of the quantal response if the assay system is such that one infectious particle can elicit the response. Unfortunately, the maximum likelihood estimate is difficult to calculate, but, by the use of a modification of Haldane's approximation, it is possible to construct a table which facilitates calculation of both the average number of infectious particles and its relative error. Additional advantages of the method are that the number of test units per dilution can be varied, the dilutions need not bear any fixed relation to each other, and the one-particle hypothesis can be readily tested.
塞利格曼，斯蒂芬·J.（加利福尼亚大学洛杉矶分校），以及M.雷·米奇。用稀释法估算传染性细菌或病毒颗粒的数量。《细菌学杂志》88:31 - 36。1964年。——对于无法获得离散感染灶的病毒或细菌系统，如果检测系统使得一个感染性颗粒能够引发反应，那么就有可能通过使用定量反应来估算感染性颗粒的数量。不幸的是，最大似然估计很难计算，但是，通过使用对霍尔丹近似法的一种改进，可以构建一个表格，便于计算感染性颗粒的平均数及其相对误差。该方法的其他优点是，每次稀释的测试单位数量可以变化，稀释倍数彼此之间不必有任何固定关系，并且单颗粒假设可以很容易地得到检验。
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