Wyshak G, Detre K
Appl Microbiol. 1972 Apr;23(4):784-90. doi: 10.1128/am.23.4.784-790.1972.
It has been demonstrated by virologists that, when procedural difficulties can be overcome and host variation can be eliminated, dosage-response data from virus assays conform with the one-particle theory of infection for both plaque counts and tissue cultures. Based on this theory, the number of virus particles from quantal virus assays can be estimated. Here a set of tables is presented from which the number of estimated particles can be obtained directly for several dilution factors and a number of dilution levels. Maximum likelihood estimation of particle counts is also illustrated using a computer program that we have prepared.
病毒学家已经证明，当程序上的困难能够被克服且宿主变异能够被消除时，病毒检测的剂量反应数据在噬斑计数和组织培养方面均符合单颗粒感染理论。基于这一理论，可以估算定量病毒检测中的病毒颗粒数量。此处给出了一组表格，通过这些表格可以直接获得针对几个稀释因子和多个稀释水平的估算颗粒数量。我们还使用编写的计算机程序展示了颗粒计数的最大似然估计。
