DONNELLY C B, BLACK L A, LEWIS K H
Appl Microbiol. 1964 Jul;12(4):311-5. doi: 10.1128/am.12.4.311-315.1964.
Samples (13) from several lots of cheddar cheese incriminated in staphylococcal food poisoning and 343 samples of cheddar cheese purchased over a 3-year period in retail markets were examined quantitatively for coagulase-positive staphylococci with the smear plate technique. Of the food-poisoning samples, 11 contained coagulase-positive staphylococci in numbers that ranged from 50 to several million per g. Of the 343 market cheese samples, 20% contained coagulase-positive staphylococci in concentrations ranging from less than 50 to more than 200,000 per g. The phage patterns of 64 of 89 cultures isolated from the food-poisoning samples placed them in the miscellaneous phage group (44A) or in phage group IV and the miscellaneous group (42D/44A); 14 had phage patterns that involved group III, the group with which food poisoning has usually been associated. In contrast, over 50% of 104 cultures from the market cheese, which were typed at 100 times the critical test dilution, had phage patterns that involved group III. Of nine selected cultures isolated from the food-poisoning cheese, three (all in phage group III) were positive for enterotoxin by intravenous injection test of cats.
对几批导致葡萄球菌食物中毒的切达干酪中的13份样本以及在零售市场3年期间购买的343份切达干酪样本，采用涂片平板技术对凝固酶阳性葡萄球菌进行了定量检测。在食物中毒样本中，11份含有凝固酶阳性葡萄球菌，数量范围为每克50至数百万个。在343份市售干酪样本中，20%含有凝固酶阳性葡萄球菌，浓度范围为每克少于50至超过200,000个。从食物中毒样本中分离出的89株培养物中的64株的噬菌体模式将它们归入杂项噬菌体组（44A）或噬菌体IV组以及杂项组（42D/44A）；14株具有涉及III组的噬菌体模式，III组通常与食物中毒有关。相比之下，在市售干酪的104株培养物中，超过50%（在临界试验稀释度的100倍下进行分型）具有涉及III组的噬菌体模式。从食物中毒干酪中分离出的9株选定培养物中，通过猫的静脉注射试验，有3株（均在噬菌体III组）肠毒素呈阳性。
