[化学敏感性试验的要点与问题，特别提及MTT法]
[Key points and problems concerning chemosensitivity tests, with special reference to MTT assay].
作者信息
Nishida M, Kohno K, Nishide K
机构信息
Department of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tsukuba, Japan.
出版信息
Hum Cell. 1992 Mar;5(1):87-98.
PMID:1419954
Abstract
Recently, the semiautomated tetrazolium-based MTT colorimetric assay have been used to measure chemosensitivity. We also have been used this assay for 4 ovarian clear cell carcinoma cell lines to investigate the chemosensitivity of this tumor. In this study, several problems have been faced to be solved. In this paper, we pointed out these problems and indicated solutions.
摘要
最近，基于四氮唑盐的半自动MTT比色法已被用于测定化学敏感性。我们也已将此检测方法用于4种卵巢透明细胞癌细胞系，以研究该肿瘤的化学敏感性。在本研究中，遇到了一些有待解决的问题。在本文中，我们指出了这些问题并给出了解决方案。
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