Harkes G, Dankert J, Feijen J
Department of Chemical Technology, University of Twente, Enschede, The Netherlands.
J Biomater Sci Polym Ed. 1992;3(5):403-18. doi: 10.1163/156856292x00213.
The adhesion and growth of two catheter-associated (O2K2 and O83K?) and two non catheter-associated (O111K58 and O157K-) uropathogenic Escherichia coli strains on glass, poly(methyl methacrylate) (PMMA), a negatively charged copolymer of MMA and methacrylic acid (MAA) and a positively charged copolymer of MMA and trimethylaminoethyl methacrylate chloride (TMAEMA-Cl) were studied. The solid surfaces were placed in a parallel plate perfusion system. After preadhesion of the bacteria onto the surfaces, growth was initiated by perfusing the system with MacConkey broth. Growth was measured by counting adherent bacteria as a function of time. Bacterial strains were characterized by means of water contact angle, microbial adhesion to hydrocarbon (MATH), anion exchange resin retention (ARR) and zeta potential measurements. Solid surfaces were characterized by means of water contact angle and zeta potential measurements. The catheter-associated strains had significantly higher water contact angles, zeta potentials and ARR values than the non catheter-associated strains. Non catheter-associated strains did not grow at the surfaces used. Catheter-associated strains did not grow at the positively charged surface but exhibited growth at the other surfaces. Strains grew more rapidly at surfaces with a relatively high negative zeta potential and a low water contact angle than at surfaces with a relatively low negative zeta potential and a high water contact angle. The growth of strain O2K2 on glass was significantly reduced when urine instead of MacConkey broth was used as perfusion medium.
研究了两种与导管相关的（O2K2和O83K？）和两种与导管无关的（O111K58和O157K-）尿路致病性大肠杆菌菌株在玻璃、聚甲基丙烯酸甲酯（PMMA）、甲基丙烯酸甲酯与甲基丙烯酸（MAA）的带负电荷共聚物以及甲基丙烯酸甲酯与甲基丙烯酰氯三甲基氨基乙酯（TMAEMA-Cl）的带正电荷共聚物上的黏附与生长情况。将固体表面置于平行板灌注系统中。在细菌预黏附到表面后，通过向系统中灌注麦康凯肉汤来启动生长。通过计算黏附细菌数量随时间的变化来测量生长情况。通过水接触角、微生物对碳氢化合物的黏附（MATH）、阴离子交换树脂保留率（ARR）和ζ电位测量对细菌菌株进行表征。通过水接触角和ζ电位测量对固体表面进行表征。与导管相关的菌株的水接触角、ζ电位和ARR值显著高于与导管无关的菌株。与导管无关的菌株在所用表面上不生长。与导管相关的菌株在带正电荷的表面上不生长，但在其他表面上表现出生长。菌株在具有相对较高负ζ电位和较低水接触角的表面上比在具有相对较低负ζ电位和较高水接触角的表面上生长得更快。当使用尿液而非麦康凯肉汤作为灌注培养基时，O2K2菌株在玻璃上的生长显著降低。
