具有不同表面润湿性和电荷的甲基丙烯酸酯聚合物对培养的人内皮细胞的黏附作用。

Adhesion of cultured human endothelial cells onto methacrylate polymers with varying surface wettability and charge.

作者信息

van Wachem P B, Hogt A H, Beugeling T, Feijen J, Bantjes A, Detmers J P, van Aken W G

机构信息

Department of Chemical Technology, Twente University of Technology, Enschede, The Netherlands.

出版信息

Biomaterials. 1987 Sep;8(5):323-8. doi: 10.1016/0142-9612(87)90001-9.

DOI:10.1016/0142-9612(87)90001-9

PMID:3676418

Abstract

The adhesion of human endothelial cells (HEC) onto a series of well-characterized methacrylate polymer surfaces with varying wettabilities and surface charges was studied either in serum-containing (CMS) or in serum-free (CM) culture medium. HEC adhesion in CMS onto (co)polymers of hydroxyethyl methacrylate (HEMA) and methyl methacrylate (MMA) was found to be optimal on the moderately wettable copolymer (mol ratio 25 HEMA/75 MMA). Positively-charged copolymers of HEMA or MMA with trimethylaminoethyl methacrylate-HCl salt (TMAEMA-Cl), both with mol ratios of 85/15 and a negatively-charged copolymer of MMA with methacrylic acid (MAA), mol ratio 85/15, showed high numbers of adhering HEC. In CM, HEC adhered onto the three charged copolymers mentioned above, but neither onto the copolymer of HEMA and MAA (mol ratio 85/15) nor onto the HEMA/MMA co- and homopolymers. Complete cell spreading in CM was only observed on the positively-charged copolymers.

摘要

研究了人内皮细胞（HEC）在一系列具有不同润湿性和表面电荷的、特性明确的甲基丙烯酸酯聚合物表面上的黏附情况，分别在含血清（CMS）或无血清（CM）培养基中进行。发现在CMS中，HEC在中等润湿性共聚物（羟乙基甲基丙烯酸酯（HEMA）与甲基丙烯酸甲酯（MMA）的摩尔比为25/75）上对（甲基丙烯酸羟乙酯（HEMA）和甲基丙烯酸甲酯（MMA）的）共聚物的黏附最佳。带正电荷的HEMA或MMA与甲基丙烯酸三甲基氨基乙酯盐酸盐（TMAEMA-Cl）的共聚物（摩尔比均为85/15），以及带负电荷的MMA与甲基丙烯酸（MAA）的共聚物（摩尔比85/15），均显示出大量黏附的HEC。在CM中，HEC黏附在上述三种带电荷的共聚物上，但不黏附在HEMA和MAA的共聚物（摩尔比85/15）以及HEMA/MMA共聚物和均聚物上。仅在带正电荷的共聚物上观察到CM中的细胞完全铺展。