Harkes G, Feijen J, Dankert J
Department of Chemical Technology, University of Twente, Enschede, The Netherlands.
Biomaterials. 1991 Nov;12(9):853-60. doi: 10.1016/0142-9612(91)90074-k.
The adhesion of three Escherichia coli strains on to six poly(methacrylates) differing in hydrophobicity and surface charge was measured as a function of time under laminar flow conditions. Polymers used were poly(methyl methacrylate) (PMMA), poly(hydroxyethyl methacrylate) (PHEMA) and copolymers of MMA or HEMA with either 15% methacrylic acid (MAA) or 15% trimethylaminoethyl methacrylate-HCl salt (TMAEMA-Cl). Bacterial and polymer surfaces were characterized by means of water contact angles and zeta potentials. Both the sessile drop contact angles and the zeta potentials of the bacterial surfaces were significantly different. No significant differences in the sessile drop contact angles of the polymer surfaces were observed. Using the Wilhelmy plate technique large contact angle hysteresis was observed for the different polymer surfaces. Surfaces of copolymers with MAA had more negative zeta potentials than those of the corresponding homopolymers. Surfaces of copolymers with TMAEMA-Cl had positive zeta potentials. The highest numbers of adherent bacteria were found on materials with positive zeta potentials, irrespective of the bacterial strain used. Bacterial adhesion on to copolymers with MAA was less than on to the corresponding homopolymers. Bacterial equilibrium adhesion values correlate with the zeta potentials of the polymer surfaces (r greater than 0.85). On substrates with less negative zeta potentials high numbers of adhered bacteria were observed. Additionally, the equilibrium bacterial adhesion values could be related with receding contact angles of polymer surfaces with negative zeta potentials (r greater than 0.86). High equilibrium adhesion values were obtained for polymers with high contact angles. No correlation between the zeta potentials and contact angles of the bacteria with the adhesion values was found.
在层流条件下，测定了三种大肠杆菌菌株在六种疏水性和表面电荷不同的聚甲基丙烯酸酯上的黏附情况，并将其作为时间的函数。所用聚合物为聚甲基丙烯酸甲酯（PMMA）、聚甲基丙烯酸羟乙酯（PHEMA）以及甲基丙烯酸甲酯（MMA）或甲基丙烯酸羟乙酯（HEMA）与15%甲基丙烯酸（MAA）或15%甲基丙烯酸三甲基氨基乙酯盐酸盐（TMAEMA-Cl）的共聚物。通过水接触角和zeta电位对细菌和聚合物表面进行了表征。细菌表面的固着滴接触角和zeta电位均存在显著差异。聚合物表面的固着滴接触角未观察到显著差异。使用Wilhelmy平板技术，观察到不同聚合物表面存在较大的接触角滞后现象。含MAA的共聚物表面的zeta电位比相应均聚物的更负。含TMAEMA-Cl的共聚物表面具有正的zeta电位。无论使用何种细菌菌株，在zeta电位为正的材料上发现的黏附细菌数量最多。细菌在含MAA的共聚物上的黏附少于在相应均聚物上的黏附。细菌平衡黏附值与聚合物表面的zeta电位相关（r大于0.85）。在zeta电位负性较小的底物上观察到大量黏附细菌。此外，平衡细菌黏附值可能与zeta电位为负的聚合物表面的后退接触角相关（r大于0.86）。对于接触角高的聚合物，获得了较高的平衡黏附值。未发现细菌的zeta电位和接触角与黏附值之间存在相关性。
