Ling E A
J Anat. 1977 Jul;123(Pt 3):637-48.
A cytochemical study of the amoeboid microglial cells in the brain of the neonatal rat has shown that these vacuolated cells exhibit strong acid phosphatase, aryl sulphatase and adenosine triphosphatase (ATPase) activities. Endogenous peroxidase, however, was not present. With the electron microscope the reaction product of acid phosphatase was found to be localized in some of the Golgi cisternae, in the majority of the electron-dense secretory granules, and in an occasional long tubular profile. The secretory granules were not uniformly stained for this enzyme, some showing only a focal reaction or none at all. The distribution of the activity of aryl sulphatase corresponded to that of acid phosphatase except that all the granules appeared to contain the former enzyme. With the light microscope the amoeboid microglial cells were intensely stained for ATPase. From these observations it was concluded that amoeboid microglia are active phagocytes and their enzyme-rich secretory granules are lysosomes.
对新生大鼠大脑中阿米巴样小胶质细胞的细胞化学研究表明，这些空泡化细胞具有很强的酸性磷酸酶、芳基硫酸酯酶和三磷酸腺苷酶（ATP酶）活性。然而，未发现内源性过氧化物酶。用电子显微镜观察发现，酸性磷酸酶的反应产物定位于一些高尔基体潴泡、大多数电子致密分泌颗粒以及偶尔出现的长管状结构中。分泌颗粒对这种酶的染色并不均匀，有些仅显示局灶性反应或根本没有反应。芳基硫酸酯酶活性的分布与酸性磷酸酶的分布相对应，只是所有颗粒似乎都含有前一种酶。在光学显微镜下，阿米巴样小胶质细胞对ATP酶呈强烈染色。从这些观察结果得出的结论是，阿米巴样小胶质细胞是活跃的吞噬细胞，其富含酶的分泌颗粒是溶酶体。
