Moore T, Keegstra K
Department of Botany, University of Wisconsin, Madison 53706.
Plant Mol Biol. 1993 Feb;21(3):525-37. doi: 10.1007/BF00028809.
Efforts to identify cDNA clones encoding chloroplastic envelope membrane proteins of Pisum sativum L. led to the isolation of a clone encoding a 92 kDa protein found in both the inner envelope membrane and the soluble fraction of chloroplasts. Sequential transcription and translation from the insert of this clone yielded a 102 kDa protein that could be imported into chloroplasts and processed to a 92 kDa form. Although the protein was identified because it reacted with antibodies to chloroplastic envelope proteins, the imported 92 kDa protein was recovered primarily in the soluble fraction of chloroplasts. The deduced amino acid sequence of this protein has strong similarity to the Clp proteins, a recently described family of highly conserved proteins present in all organisms examined to date. The physiological significance of the presence of this protein in chloroplasts is discussed.
为鉴定编码豌豆叶绿体被膜蛋白的cDNA克隆所做的努力，导致分离出一个编码92 kDa蛋白的克隆，该蛋白存在于内膜和叶绿体的可溶部分。从该克隆的插入片段进行连续转录和翻译，产生了一个102 kDa的蛋白，该蛋白可被导入叶绿体并加工成92 kDa的形式。尽管该蛋白是因为它与叶绿体被膜蛋白抗体发生反应而被鉴定出来的，但导入的92 kDa蛋白主要在叶绿体的可溶部分中回收。该蛋白推导的氨基酸序列与Clp蛋白有很强的相似性，Clp蛋白是最近描述的一个高度保守的蛋白家族，迄今为止在所有检测过的生物体中都存在。本文讨论了该蛋白在叶绿体中存在的生理意义。
