用荧光抗体检测鸡蛋和蛋制品中的沙门氏菌
DETECTION OF SALMONELLA IN EGGS AND EGG PRODUCTS WITH FLUORESCENT ANTIBODY.
作者信息
HAGLUND J R, AYRES J C, PATON A M, KRAFT A A, QUINN L Y
出版信息
Appl Microbiol. 1964 Sep;12(5):447-50. doi: 10.1128/am.12.5.447-450.1964.
Abstract
Organisms of the genus Salmonella are detected in eggs and egg products within 24 hr in the presence of Pseudomonadaceae and other Enterobacteriaceae by combining selective cultural methods with fluorescent-antibody techniques. These techniques are specific for Salmonella when H antibodies are used. Absorption techniques are necessary before the O antibodies give specific reactions for Salmonella. No cross-reactions appear when H antiserum is used. Absorption and interference techniques indicate the test is specific for Salmonella.
摘要
通过将选择性培养方法与荧光抗体技术相结合，在假单胞菌科和其他肠杆菌科存在的情况下，24小时内可在鸡蛋和蛋制品中检测到沙门氏菌属的微生物。当使用H抗体时，这些技术对沙门氏菌具有特异性。在O抗体对沙门氏菌产生特异性反应之前，吸收技术是必要的。使用H抗血清时不会出现交叉反应。吸收和干扰技术表明该测试对沙门氏菌具有特异性。
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