通气及亚硒酸盐延迟添加法在沙门氏菌分离中的适用性
Applicability of aeration and delayed addition of selenite to the isolation of salmonellae.
作者信息
Alford J A, Knight N L
出版信息
Appl Microbiol. 1969 Dec;18(6):1060-4. doi: 10.1128/am.18.6.1060-1064.1969.
Abstract
The effect of aeration combined with the delayed addition of selenite on the lag period of several strains of salmonellae and other enterobacteria is reported. A procedure has been developed involving shaking of the sample in a basal medium for 4 hr at 37 C, adding selenite and cystine, and continuing shaking for 20 hr. Confirmation by selective plating, biochemical tests, and serology gave results comparable to the standard lactose pre-enrichment method with the saving of 24 hr and elimination of one set of media. Confirmation by fluorescent-antibody tests showed that fewer positive fluorescent stains were obtained from the aerated procedure than from the lactose pre-enrichment procedure. Precautions in the application of this procedure are discussed.
摘要
报道了通气结合亚硒酸盐延迟添加对几种沙门氏菌菌株和其他肠道杆菌滞后期的影响。已开发出一种方法，即将样品在基础培养基中于37℃振荡4小时，加入亚硒酸盐和胱氨酸，然后继续振荡20小时。通过选择性平板培养、生化试验和血清学进行的验证，其结果与标准乳糖预富集方法相当，节省了24小时并省去了一组培养基。荧光抗体试验的验证表明，与乳糖预富集方法相比，通气方法获得的阳性荧光染色较少。文中讨论了该方法应用中的注意事项。
相似文献
1
Applicability of aeration and delayed addition of selenite to the isolation of salmonellae.通气及亚硒酸盐延迟添加法在沙门氏菌分离中的适用性
Appl Microbiol. 1969 Dec;18(6):1060-4. doi: 10.1128/am.18.6.1060-1064.1969.
2
Comparison of several methods for the isolation of salmonellae from egg products.几种从蛋制品中分离沙门氏菌方法的比较。
Can J Microbiol. 1975 Jul;21(7):1041-5. doi: 10.1139/m75-154.
3
A comparison of strontium selenite broth and mannitol-selenite-cystine broth in the isolation of salmonellae from egg products.亚硒酸锶肉汤与亚硒酸盐-胱氨酸-甘露醇肉汤用于从蛋制品中分离沙门氏菌的比较
Aust J Exp Biol Med Sci. 1975 Aug;53(4):329-31. doi: 10.1038/icb.1975.36.
4
Salmonellae as an index of pollution of surface waters.沙门氏菌作为地表水污染指标。
Appl Microbiol. 1972 Sep;24(3):334-40. doi: 10.1128/am.24.3.334-340.1972.
5
[A comparison of methods for the enrichment of Salmonellae from human feces].[从人类粪便中富集沙门氏菌的方法比较]
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1982 Nov;253(2):183-96.
6
Form of selenium in selenite enrichment media for isolation of salmonellae.用于分离沙门氏菌的亚硒酸盐富集培养基中硒的形态。
Appl Microbiol. 1974 Apr;27(4):814-6. doi: 10.1128/am.27.4.814-816.1974.
7
Comparison of enrichment procedures for fluorescent antibody and cultural detection of salmonellae in raw meat and poultry.生肉和家禽中沙门氏菌荧光抗体富集程序与培养检测的比较
Appl Environ Microbiol. 1976 May;31(5):787-8. doi: 10.1128/aem.31.5.787-788.1976.
8
ISOLATION OF SALMONELLAE FROM FOOD SAMPLES. VI. COMPARISON OF METHODS FOR THE ISOLATION OF SALMONELLA FROM EGG PRODUCTS.从食品样本中分离沙门氏菌。六、从蛋制品中分离沙门氏菌的方法比较。
Appl Microbiol. 1964 May;12(3):224-8. doi: 10.1128/am.12.3.224-228.1964.
9
Effect of type of enrichment and duration of incubation on salmonella recovery from meat-and-bone meal.富集类型和培养时间对从肉骨粉中回收沙门氏菌的影响。
Appl Microbiol. 1972 Mar;23(3):578-85. doi: 10.1128/am.23.3.578-585.1972.
10
Growth kinetics of mixed culture in salmonella enrichment media.沙门氏菌增菌培养基中混合培养物的生长动力学
J Appl Bacteriol. 1985 Sep;59(3):231-7. doi: 10.1111/j.1365-2672.1985.tb01784.x.
引用本文的文献
1
Rapid detection of sublethally impaired cells of Enterobacteriaceae in dried foods.快速检测干制食品中肠杆菌科亚致死损伤细胞
Appl Microbiol. 1970 Aug;20(2):273-5. doi: 10.1128/am.20.2.273-275.1970.
2
Increased recovery of salmonellae from environmental samples enriched with buffered peptone water.用缓冲蛋白胨水增菌的环境样本中沙门氏菌回收率增加。
Appl Environ Microbiol. 1977 Sep;34(3):270-3. doi: 10.1128/aem.34.3.270-273.1977.
本文引用的文献
1
Immunofluorescence technique for the detection of salmonellae in various foods.用于检测各类食品中沙门氏菌的免疫荧光技术。
Appl Microbiol. 1967 Sep;15(5):1145-9. doi: 10.1128/am.15.5.1145-1149.1967.
2
DETECTION OF SALMONELLA IN EGGS AND EGG PRODUCTS WITH FLUORESCENT ANTIBODY.用荧光抗体检测鸡蛋和蛋制品中的沙门氏菌
Appl Microbiol. 1964 Sep;12(5):447-50. doi: 10.1128/am.12.5.447-450.1964.
3
OCCURRENCE OF GRAM-POSITIVE ORGANISMS POSSESSING CHARACTERISTICS SIMILAR TO THOSE OF SALMONELLA AND THE PRACTICAL PROBLEM OF RAPID AND DEFINITIVE SALMONELLA IDENTIFICATION.具有与沙门氏菌相似特征的革兰氏阳性菌的出现以及沙门氏菌快速准确鉴定的实际问题。
Appl Microbiol. 1964 Sep;12(5):395-9. doi: 10.1128/am.12.5.395-399.1964.
4
Regeneration of ribosomes and ribosomal ribonucleic acid during repair of thermal injury to Staphylococcus.葡萄球菌热损伤修复过程中核糖体及核糖体核糖核酸的再生
J Bacteriol. 1967 Oct;94(4):1082-7. doi: 10.1128/jb.94.4.1082-1087.1967.
5
Enrichment medium for selection of Salmonella from fish homogenate.用于从鱼匀浆中筛选沙门氏菌的富集培养基。
Appl Microbiol. 1966 Jan;14(1):12-20. doi: 10.1128/am.14.1.12-20.1966.
6
Repair of thermal injury of Staphylococcus aureus.金黄色葡萄球菌热损伤的修复
J Bacteriol. 1966 Jan;91(1):134-42. doi: 10.1128/jb.91.1.134-142.1966.
7
Inhibitory action of selenite on Escherichia coli, Proteus vulgaris, and Salmonella thompson.亚硒酸盐对大肠杆菌、普通变形杆菌和汤普森沙门氏菌的抑制作用。
J Bacteriol. 1965 Oct;90(4):857-62. doi: 10.1128/jb.90.4.857-862.1965.
8
Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions.仅涉及肉汤培养和血清学反应的干制食品和饲料中沙门氏菌检测的加速程序。
Appl Microbiol. 1969 Apr;17(4):533-9. doi: 10.1128/am.17.4.533-539.1969.
9
A new plating medium for the isolation of enteric pathogens. I. hektoen enteric agar.一种用于分离肠道病原体的新型平板培养基。I. 赫氏肠道琼脂培养基
Appl Microbiol. 1968 Apr;16(4):577-8. doi: 10.1128/am.16.4.577-578.1968.
10
Antimicrobial effect of cocoa on salmonellae.可可对沙门氏菌的抗菌作用。
Appl Microbiol. 1968 Feb;16(2):424-5. doi: 10.1128/am.16.2.424-425.1968.
Zotero 插件