用于检测各类食品中沙门氏菌的免疫荧光技术。
Immunofluorescence technique for the detection of salmonellae in various foods.
作者信息
Insalata N F, Schulte S J, Berman J H
机构信息
General Foods Technical Center, Tarrytown, New York 10591.
出版信息
Appl Microbiol. 1967 Sep;15(5):1145-9. doi: 10.1128/am.15.5.1145-1149.1967.
Abstract
Salmonella species have been detected in nine food varieties by use of fluorescent antibodies without false-positive or false-negative results. Test antisera were specially prepared, commercially available, conjugated polyvalent O globulin absorbed with cultures of Escherichia coli and Citrobacter freundii, and polyvalent phase II H globulin antibodies. Use of this technique permits a decrease of 24 hr in time normally required for Salmonella detection when compared with cultural Salmonella recovery methods.
摘要
利用荧光抗体在九种食品中检测出了沙门氏菌，且无假阳性或假阴性结果。检测抗血清是专门制备的，有市售产品，即经大肠杆菌和弗氏柠檬酸杆菌培养物吸收的结合多价O球蛋白，以及多价II相H球蛋白抗体。与沙门氏菌培养回收方法相比，使用该技术可将沙门氏菌检测的正常所需时间减少24小时。
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2
A RAPID IMMUNOFLUORESCENCE TECHNIQUE FOR DETECTING SALMONELLAE IN RAW MEAT.
J Hyg (Lond). 1964 Sep;62(3):319-27. doi: 10.1017/s0022172400040031.
3
Use of crystal violet or brilliant green dyes for the determination of Salmonellae in dried food products.
J Bacteriol. 1960 Dec;80(6):861. doi: 10.1128/jb.80.6.861-861.1960.
4
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Appl Microbiol. 1961 May;9(3):188-95. doi: 10.1128/am.9.3.188-195.1961.
5
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J Bacteriol. 1959 Apr;77(4):478-86. doi: 10.1128/jb.77.4.478-486.1959.
6
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