Robertson C R, Pisetsky D S
Division of Rheumatology and Immunology, Duke University Medical Center, Durham, N.C.
Int Arch Allergy Immunol. 1992;98(4):311-6. doi: 10.1159/000236204.
To assess the role of infection in anti-DNA antibody production, the DNA-binding activity of sera from patients with Escherichia coli bacteremia was analyzed. Among 8 patients with bacteremia documented by blood culture, 5 demonstrated increased levels of antibodies to single-stranded DNA from E. coli as measured by enzyme-linked immunosorbent assay. Sera from these patients also reacted with single-stranded DNA from other bacterial and mammalian species as well as certain synthetic polynucleotides including poly-dT and poly-dC. The isotype distribution of these antibodies and their avidity as assessed by competition enzyme-linked immunosorbent assay resembled, moreover, responses of patients with systemic lupus erythematosus. These results suggest that, during the course of infection with E. coli, some patients may produce antibodies with immunochemical properties similar to those arising in systemic lupus erythematosus.
为评估感染在抗DNA抗体产生中的作用，分析了大肠杆菌菌血症患者血清的DNA结合活性。在8例血培养证实有菌血症的患者中，5例通过酶联免疫吸附测定显示抗大肠杆菌单链DNA抗体水平升高。这些患者的血清还与来自其他细菌和哺乳动物物种的单链DNA以及某些合成多核苷酸（包括聚dT和聚dC）发生反应。此外，通过竞争酶联免疫吸附测定评估的这些抗体的同种型分布及其亲和力类似于系统性红斑狼疮患者的反应。这些结果表明，在大肠杆菌感染过程中，一些患者可能产生具有与系统性红斑狼疮中产生的抗体相似免疫化学特性的抗体。
