Ruffatti A, Peserico A, Rondinone R, Calligaro A, Del Ross T, Ghirardello A, Germino M, Todesco S
Division of Rheumatology, University of Padova, Italy.
Arch Dermatol. 1991 Aug;127(8):1180-3.
Prevalence, levels, and immunoglobulin classes of anti-single-stranded DNA antibodies were determined by an enzyme-linked immunosorbent assay in 52 patients with localized scleroderma (33 with morphea, four with generalized morphea, and 15 with linear scleroderma), in 60 healthy controls, and, for comparison, in 31 patients with systemic lupus erythematosus. Localized scleroderma revealed a significant prevalence of anti-single-stranded DNA antibodies, mainly characterized by high levels and IgM and IgA isotypes. Comparison of antibody characteristics in different clinical forms of localized scleroderma showed some significant differences (levels and immunoglobulin isotypes). Comparison with systemic lupus erythematosus showed that frequency, high levels, and IgG isotype of anti-single-stranded DNA antibodies significantly prevailed in systemic lupus erythematosus, while the IgM isotype significantly prevailed in localized scleroderma. However, generalized morphea and linear scleroderma did not significantly differ from systemic lupus erythematosus as regards antibody frequency and prevalence of high antibody levels.
采用酶联免疫吸附测定法，对52例局限性硬皮病患者（33例斑块状硬皮病、4例泛发性斑块状硬皮病和15例线状硬皮病）、60例健康对照者以及31例系统性红斑狼疮患者进行检测，以确定抗单链DNA抗体的患病率、水平及免疫球蛋白类别。局限性硬皮病患者中抗单链DNA抗体患病率显著升高，主要表现为高水平以及IgM和IgA亚型。不同临床类型局限性硬皮病抗体特征的比较显示出一些显著差异（水平和免疫球蛋白亚型）。与系统性红斑狼疮比较发现，系统性红斑狼疮中抗单链DNA抗体的频率、高水平及IgG亚型显著占优，而IgM亚型在局限性硬皮病中显著占优。然而，泛发性斑块状硬皮病和线状硬皮病在抗体频率和高抗体水平患病率方面与系统性红斑狼疮并无显著差异。
