Covert S F, Bolduc J, Cullen D
Department of Bacteriology, University of Wisconsin-Madison 53706.
Curr Genet. 1992 Nov;22(5):407-13. doi: 10.1007/BF00352442.
Southern blot and nucleotide sequence analysis of Phanerochaete chrysosporium BKM-F-1767 genomic clones indicate that this wood-degrading fungus contains at least six genes with significant homology to the Trichoderma reesei cellobiohydrolase I gene (cbh1). Using pulsed-field gel electrophoresis to separate P. chrysosporium chromosomes, the six cellulase genes were found to hybridize to at least three different chromosomes, one of which is dimorphic. The organization of these genes was similar in another P. chrysosporium strain, ME 446. It is clear that, unlike T. reesei, the most well-studied cellulolytic fungus, P. chrysosporium contains a complex, cbh1-like gene family.
对黄孢原毛平革菌BKM-F-1767基因组克隆进行的Southern杂交和核苷酸序列分析表明，这种木材降解真菌含有至少六个与里氏木霉纤维二糖水解酶I基因（cbh1）具有显著同源性的基因。利用脉冲场凝胶电泳分离黄孢原毛平革菌的染色体，发现这六个纤维素酶基因与至少三条不同的染色体杂交，其中一条是双态的。在黄孢原毛平革菌的另一个菌株ME 446中，这些基因的组织方式相似。显然，与研究最深入的纤维素分解真菌里氏木霉不同，黄孢原毛平革菌含有一个复杂的、类似cbh1的基因家族。
