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Genomic organization of lignin peroxidase genes of Phanerochaete chrysosporium.

作者信息

Gaskell J, Dieperink E, Cullen D

机构信息

Institute for Microbial and Biochemical Technology, Forest Products Laboratory, Madison, WI 53705.

出版信息

Nucleic Acids Res. 1991 Feb 11;19(3):599-603. doi: 10.1093/nar/19.3.599.

DOI:10.1093/nar/19.3.599
PMID:2011531
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC333654/
Abstract

Three lignin peroxidase (LiP) genes from the basidiomycete Phanerochaete chrysosporium were cloned on a single 30 kb cosmid insert. One gene, GLG5, is the genomic equivalent of a previously reported cDNA clone, CLG5. The other two LiP genes are transcriptionally convergent and map to a position approximately 15 kb downstream of GLG5. The translational stop codons of these genes are separated by 1.3 kb. Analysis of homokaryons established allelic relationships to previously described LiP clones. Using clamped homogeneous electrical field electrophoresis (CHEF), seven chromosomal bands were resolved from P. chrysosporium genomic DNA. On CHEF gel Southern blots, the LiP gene family was localized to a single, dimorphic chromosome.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4224/333654/6be6a952cc4a/nar00239-0178-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4224/333654/92be01be7f8a/nar00239-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4224/333654/ba15134f93f7/nar00239-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4224/333654/ef00a63f9fec/nar00239-0178-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4224/333654/6be6a952cc4a/nar00239-0178-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4224/333654/92be01be7f8a/nar00239-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4224/333654/ba15134f93f7/nar00239-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4224/333654/ef00a63f9fec/nar00239-0178-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4224/333654/6be6a952cc4a/nar00239-0178-c.jpg

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