Shimizu N, Tomoda M, Takada K, Gonda R
Kyoritsu College of Pharmacy, Tokyo, Japan.
Chem Pharm Bull (Tokyo). 1992 Aug;40(8):2125-8. doi: 10.1248/cpb.40.2125.
The controlled Smith degradation and limited hydrolysis of glycyrrhizan UA, the main phagocytosis-activating polysaccharide isolated from the root of Glycyrrhiza uralensis FISCHER, was performed. The reticuloendothelial system-potentiating, anti-complementary and alkaline phosphatase-inducing activities of glycyrrhizan UA and its degradation products were investigated. Methylation analyses of primary, secondary and tertiary Smith degradation products and of the limited hydrolysis product indicated that the core structural features of glycyrrhizan UA include a backbone chain composed of beta-1,3-linked D-galactose. All of the galactose units in the backbone carry side chains composed of mainly alpha-1,5-linked L-arabino-beta-1,6- or 1,3-linked D-galactose residues at position 6. Removal of the arabinosyl side chains caused a pronounced decrease in immunological activity.
对从乌拉尔甘草根部分离出的主要促吞噬活性多糖甘草酸 UA 进行了可控的史密斯降解和有限水解。研究了甘草酸 UA 及其降解产物的网状内皮系统增强、抗补体和碱性磷酸酶诱导活性。对一级、二级和三级史密斯降解产物以及有限水解产物的甲基化分析表明，甘草酸 UA 的核心结构特征包括由 β-1,3-连接的 D-半乳糖组成的主链。主链中的所有半乳糖单元在 6 位带有主要由 α-1,5-连接的 L-阿拉伯糖基-β-1,6-或 1,3-连接的 D-半乳糖残基组成的侧链。去除阿拉伯糖基侧链会导致免疫活性显著降低。
