Tomoda M, Hirabayashi K, Shimizu N, Gonda R, Ohara N
Kyoritsu College of Pharmacy, Tokyo, Japan.
Biol Pharm Bull. 1994 Sep;17(9):1287-91. doi: 10.1248/bpb.17.1287.
Controlled Smith degradation and limited hydrolysis of ginsenan PA, the main phagocytosis-activating polysaccharide isolated from the root of Panax ginseng C. A. Meyer, were performed. The reticuloendothelial system-potentiating and anti-complementary activities of the degradation products were investigated. Methylation analysis of the primary and secondary Smith degradation products indicated that the core structural features of ginsenan PA include a backbone chain mainly composed of beta-1,3-linked D-galactose. Almost half of the galactose units in the backbone carry side-chains composed of beta-1,6-linked D-galactosyl residues at position 6. Further 3,6-branching of D-galactose units was observed in a part of the side-chains. alpha-L-Arabinose units are connected mainly to the core galactose moieties via position 6. Removal of most of the arabinose units had a considerable effect on immunological activity.
对从人参（Panax ginseng C. A. Meyer）根部分离出的主要促吞噬活性多糖人参聚糖PA进行了可控的史密斯降解和有限水解。研究了降解产物的网状内皮系统增强活性和抗补体活性。对一级和二级史密斯降解产物的甲基化分析表明，人参聚糖PA的核心结构特征包括一条主要由β-1,3-连接的D-半乳糖组成的主链。主链中几乎一半的半乳糖单元在6位带有由β-1,6-连接的D-半乳糖基残基组成的侧链。在部分侧链中观察到D-半乳糖单元的进一步3,6-分支。α-L-阿拉伯糖单元主要通过6位连接到核心半乳糖部分。去除大部分阿拉伯糖单元对免疫活性有相当大的影响。
