Kiho T, Shiose Y, Nagai K, Sakushima M, Ukai S
Gifu Pharmaceutical University, Japan.
Chem Pharm Bull (Tokyo). 1992 Aug;40(8):2212-4. doi: 10.1248/cpb.40.2212.
An antitumor polysaccharide containing peptide moieties AT-HW ([alpha]D + 31 degrees in water) and an antitumor polysaccharide AT-AL ([alpha]D + 209 degrees in 1 M sodium hydroxide) were isolated from hot-water extract and the alkaline extract of the fruiting bodies of Armillariella tabescens, respectively. Chemical structures of AT-HW and AT-AL were investigated by a combination of chemical and spectroscopic methods. The results indicate that the major constituent of AT-HW (molecular weight, 105000), a heteroglycan, is composed primarily of beta-(1----6)-linked D-glucopyranosyl and D-galactopyranosyl residues, and contains their branched residues and terminal sugar (gluco-, manno-, and fucopyranose) residues, in addition to beta-(1----3)-linked D-glucopyranosyl residues, while AT-AL (molecular weight, 93000) is chiefly composed of alpha-(1----3)-linked D-glucopyranosyl residues.
从亮菌子实体的热水提取物和碱提取物中分别分离出含肽部分的抗肿瘤多糖AT-HW（在水中[α]D + 31°）和抗肿瘤多糖AT-AL（在1 M氢氧化钠中[α]D + 209°）。通过化学和光谱方法相结合对AT-HW和AT-AL的化学结构进行了研究。结果表明，AT-HW（分子量105000）的主要成分是一种杂聚糖，主要由β-(1→6)-连接的D-吡喃葡萄糖基和D-吡喃半乳糖基残基组成，除了β-(1→3)-连接的D-吡喃葡萄糖基残基外，还含有它们的分支残基和末端糖（葡萄糖、甘露糖和岩藻糖）残基，而AT-AL（分子量93000）主要由α-(1→3)-连接的D-吡喃葡萄糖基残基组成。
