Dietrich F M
Immunology. 1966 Apr;10(4):365-76.
Several parameters such as dosage and schedule of antigen injection were examined. The effect of a single intraperitoneal or intravenous injection of various amounts of sheep red cells (SRBC) was studied over a dose range of more than 10. Approximately 5×10 SRBC was the optimal amount of antigen beyond which no further increase in titre occurred. Multiple injections and overloading the organism with large amounts of SRBC did not result in a depression of the immune response. Immunization with SRBC incorporated into incomplete Freund's adjuvant was slightly inferior to immunization with SRBC suspended in saline. The response depended on the antigen concentration in the adjuvant mixture rather than on the absolute amount of antigen injected. Early in immunization antibodies against SRBC belong to the 19S class and change gradually into 7S antibodies. 19S and 7S haemolytic activity was mercaptoethanol-sensitive. From absorption experiments it was concluded that the mouse antibody against SRBC has specificities other than anti-Forssman, anti-mononucleosis and anti-serum sickness and is directed against an isophilic heat-labile antigen(s).
研究了几个参数，如抗原注射的剂量和时间表。在超过10倍的剂量范围内，研究了单次腹腔内或静脉内注射不同量绵羊红细胞（SRBC）的效果。约5×10个SRBC是最佳抗原量，超过此量滴度不再进一步增加。多次注射以及用大量SRBC使机体超载并未导致免疫反应受到抑制。用不完全弗氏佐剂包被的SRBC进行免疫略逊于用悬浮于盐水中的SRBC进行免疫。反应取决于佐剂混合物中的抗原浓度，而非注射抗原的绝对量。免疫早期，针对SRBC的抗体属于19S类，并逐渐转变为7S抗体。19S和7S溶血活性对巯基乙醇敏感。从吸收实验得出结论，小鼠抗SRBC抗体具有除抗福斯曼、抗单核细胞增多症和抗血清病之外的特异性，且针对一种嗜异性热不稳定抗原。
