Chevalier X, Groult N, Labat-Robert J
Laboratoire de Biochimie du Tissu Conjonctif, Creteil, France.
Clin Physiol Biochem. 1992;9(1):1-6.
The biosynthesis of fibronectin was determined in explants from normal and osteoarthritic human cartilage after metabolic labeling and immunoprecipitation. Each sample of osteoarthritic cartilage was divided into three regions taken at different distances from the eburnated bone area. Only full depth cartilage samples were taken into consideration. We could detect a low level of fibronectin biosynthesis in normal cartilage. In osteoarthritic cartilage increased synthesis of fibronectin was demonstrated, the most important in the region close to the eburnated area. Increased synthesis, although to a lower extent, was also demonstrated in the two other regions at increasing distances from the eburnated areas. Immuno-histological examinations performed on tissue samples and similar studies on articular chondrocyte cell cultures confirmed the accumulation of newly synthesized fibronectin in pathological conditions.
在进行代谢标记和免疫沉淀后，测定了正常和骨关节炎人类软骨外植体中纤连蛋白的生物合成。每个骨关节炎软骨样本被分为距骨侵蚀区不同距离的三个区域。仅考虑全层软骨样本。我们可以检测到正常软骨中纤连蛋白生物合成水平较低。在骨关节炎软骨中，纤连蛋白的合成增加，在靠近骨侵蚀区的区域最为明显。在距骨侵蚀区距离增加的另外两个区域也显示出合成增加，尽管程度较低。对组织样本进行的免疫组织学检查以及对关节软骨细胞培养物的类似研究证实了病理条件下新合成的纤连蛋白的积累。
