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链球菌细胞壁研究。VII. B族细胞壁的碳水化合物组成。

STUDIES OF STREPTOCOCCAL CELL WALLS. VII. CARBOHYDRATE COMPOSITION OF GROUP B CELL WALLS.

作者信息

WITTNER M K, HAYASHI J A

出版信息

J Bacteriol. 1965 Feb;89(2):398-402. doi: 10.1128/jb.89.2.398-402.1965.

DOI:10.1128/jb.89.2.398-402.1965
PMID:14255706
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC305520/
Abstract

Wittner, Masako K. (Presbyterian-St. Luke's Hospital, Chicago, Ill.), and James A. Hayashi. Studies of streptococcal cell walls. VII. Carbohydrate composition of group B cell walls. J. Bacteriol. 89:398-402. 1965.-Group B streptococcal cell walls contain 63% protein, 10% rhamnose, 18% hexose (mainly galactose, but also some glucose), 7% hexosamine (mainly glucosamine, but also galactosamine), and 3% muramic acid. The group and type antigens were extracted from isolated cell walls by acid treatment and enzymatic hydrolysis, and fractionated either with ethanol or on a diethylaminoethyl-cellulose column. Serological and chemical analyses of the fractions obtained in the two fractionation methods show that the group antigen is a rhamnose-rich polysaccharide and that the type antigen is rich in galactose and contains hexosamines.

摘要

威特纳,正子·K.(长老会-圣卢克医院,伊利诺伊州芝加哥),以及詹姆斯·A.林。链球菌细胞壁的研究。VII. B族细胞壁的碳水化合物组成。《细菌学杂志》89:398 - 402。1965年。- B族链球菌细胞壁含有63%的蛋白质、10%的鼠李糖、18%的己糖(主要是半乳糖,但也有一些葡萄糖)、7%的己糖胺(主要是葡糖胺,但也有半乳糖胺)和3%的胞壁酸。通过酸处理和酶水解从分离的细胞壁中提取群抗原和型抗原,并用乙醇或在二乙氨基乙基纤维素柱上进行分级分离。对两种分级分离方法得到的级分进行血清学和化学分析表明,群抗原是一种富含鼠李糖的多糖,型抗原富含半乳糖并含有己糖胺。

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A spectrophotometric method for the microdetermination of hexosamines.一种用于微量测定己糖胺的分光光度法。
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Studies of streptococcal cell walls. III. The amino acids of the trypsin-treated cell wall.链球菌细胞壁的研究。III. 经胰蛋白酶处理的细胞壁中的氨基酸。
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