Javadpour N, Guirguis R
University of Maryland, Baltimore 21201.
Eur Urol. 1992;21 Suppl 1:1-4. doi: 10.1159/000474877.
We have studied the role of combined measurements of tumor collagenase-stimulating factor (TCSF) and tumor autocrine motility factor (TAMF) in 83 first morning voids and 24-hour specimens in patients with bladder and renal cancer and control subjects. TCSF and TAMF were measured by immunoabsorption assay and motility test utilizing modified Boyden chamber. The mean concentrations of urinary TCSF and TAMF from cancer patients (n = 32) were 4- to 5-fold higher than those from benign conditions (n = 70). We compared TCSF and TAMF utilizing motility test in first morning voids (n = 18) and 24-hour urinary samples (n = 19). TCSF and TAMF were almost identical in terms of eluted proteins from morning voids and 24-hour urine samples. Invasive bladder cancer (n = 12) showed a significantly higher correlation (r = 0.9 and p = 0.0001) between motility response and urinary concentration of TCSF and TAMF. We have also localized these two glycoproteins in cell membranes and cytoplasms of cancer cell.
我们研究了肿瘤胶原酶刺激因子（TCSF）和肿瘤自分泌运动因子（TAMF）联合检测在膀胱癌和肾癌患者及对照受试者的83份首次晨尿和24小时样本中的作用。通过免疫吸附测定和利用改良博伊登小室的运动试验来检测TCSF和TAMF。癌症患者（n = 32）尿液中TCSF和TAMF的平均浓度比良性疾病患者（n = 70）高4至5倍。我们在首次晨尿（n = 18）和24小时尿液样本（n = 19）中利用运动试验比较了TCSF和TAMF。就晨尿和24小时尿液样本中的洗脱蛋白而言，TCSF和TAMF几乎相同。浸润性膀胱癌（n = 12）在运动反应与尿液中TCSF和TAMF浓度之间显示出显著更高的相关性（r = 0.9，p = 0.0001）。我们还将这两种糖蛋白定位在癌细胞的细胞膜和细胞质中。
