氧气供应速率对大肠杆菌生长的影响。
EFFECT OF OXYGEN SUPPLY RATES ON GROWTH OF ESCHERICHIA COLI.
作者信息
MCDANIEL L E, BAILEY E G, ZIMMERLI A
出版信息
Appl Microbiol. 1965 Jan;13(1):109-14. doi: 10.1128/am.13.1.109-114.1965.
The effect of oxygen-supply rates on bacterial growth was studied in commercially available unbaffled and baffled flasks with the use of Escherichia coli in a synthetic medium as a test system. The amount of growth obtained depended on the oxygen-supply rate. Based on oxygen-absorption rates (OAR) measured by the rate of sulfite oxidation, equal OAR values in different types of flasks did not give equal amounts of growth. However, growth was essentially equal at the equal sulfite-oxidation rates when these were determined in the presence of killed whole cultures. Specific growth rates were reduced only at oxygen-supply rates much lower than those at which the total amount of growth was reduced. For the physical set-up used in this work and with the biological system employed, Bellco 598 flasks and flasks fitted with Biotech stainless-steel baffles gave satisfactory results at workable broth volumes; unbaffled and Bellco 600 flasks did not.
以合成培养基中的大肠杆菌为测试系统，在市售的无挡板和有挡板烧瓶中研究了氧气供应速率对细菌生长的影响。获得的生长量取决于氧气供应速率。根据通过亚硫酸盐氧化速率测量的氧气吸收率（OAR），不同类型烧瓶中相等的OAR值并未产生相等的生长量。然而，当在灭活的全培养物存在下测定亚硫酸盐氧化速率时，在相等的亚硫酸盐氧化速率下生长基本相等。特定生长速率仅在氧气供应速率远低于生长总量降低时的速率时才会降低。对于本研究中使用的物理装置和所采用的生物系统，Bellco 598烧瓶和装有Biotech不锈钢挡板的烧瓶在可行的肉汤体积下给出了令人满意的结果；无挡板烧瓶和Bellco 600烧瓶则不然。
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