McDaniel L E, Bailey E G
Appl Microbiol. 1969 Feb;17(2):286-90. doi: 10.1128/am.17.2.286-290.1969.
Growth of microorganisms and biosynthesis of microbial products in shake flasks may be limited by operating conditions which provide inadequate supplies of oxygen. Methods are described for meeting the oxygen requirements of test organisms by using standard baffled flasks with pad-type closures and shaking at relatively high speeds. Growth of Escherichia coli B in a rich medium and production of candidin by Streptomyces viridoflavus were the test systems used. Flasks shaken at 230 to 385 rev/min gave sulfite oxidation rates of 1 to 8 mmoles of oxygen per liter per min over a useful working volume range (40 to 150 ml in 300-ml flasks). These rates are as high as those obtained in agitated fermentors under usual operating conditions.
摇瓶中微生物的生长和微生物产物的生物合成可能会受到操作条件的限制，这些条件提供的氧气供应不足。本文描述了通过使用带有垫式瓶盖的标准挡板摇瓶并以相对较高的速度振荡来满足受试生物氧气需求的方法。使用的测试系统是在丰富培养基中大肠杆菌B的生长以及绿色链霉菌产生杀假丝菌素。在230至385转/分钟振荡的摇瓶在有用的工作体积范围内（300毫升摇瓶中为40至150毫升）产生的亚硫酸盐氧化速率为每分钟每升1至8毫摩尔氧气。这些速率与在常规操作条件下搅拌发酵罐中获得的速率一样高。
