供氧速率对大肠杆菌生长的影响。II. 摇瓶和50升发酵罐中的结果比较。
EFFECT OF OXYGEN-SUPPLY RATES ON GROWTH OF ESCHERICHIA COLI. II. COMPARISON OF RESULTS IN SHAKE FLASKS AND 50-LITER FERMENTOR.
作者信息
MCDANIEL L E, BAILEY E G, ZIMMERLI A
出版信息
Appl Microbiol. 1965 Jan;13(1):115-9. doi: 10.1128/am.13.1.115-119.1965.
Growth of Escherichia coli and chemical changes in the medium were very similar in highly baffled flasks and in a 50-liter fermentor run under the same oxygen-supply conditions, based on sulfite-oxidation rates. Flasks with stainless-steel baffles (Biotech) gave growth patterns and rates of glucose and NH(4)-N utilization almost identical to those of the fermentor; results with Bellco 598 flasks (with 6 to 7 mm deep indentations) were quite similar. Unbaffled and Bellco 600 flasks (3 to 4 mm indentations) were similar to the fermentor at very high and very low oxygen-transfer rates, but gave much less growth than the fermentor at intermediate levels. Maximal oxygen-uptake rates occurred in the fermentor at the end of the logarithmic-growth phase when growth was 40 to 75% of maximum. In the fermentor, both sulfite-oxidation rates and rates of oxygen uptake correlated reasonably well with the total amount of growth produced.
基于亚硫酸盐氧化速率，在相同氧气供应条件下，高度 baffled 烧瓶和 50 升发酵罐中大肠杆菌的生长以及培养基中的化学变化非常相似。带有不锈钢 baffles（生物技术公司）的烧瓶产生的生长模式以及葡萄糖和 NH(4)-N 的利用速率几乎与发酵罐相同；Bellco 598 烧瓶（有 6 至 7 毫米深的凹痕）的结果非常相似。无 baffles 的烧瓶和 Bellco 600 烧瓶（3 至 4 毫米凹痕）在非常高和非常低的氧气传递速率下与发酵罐相似，但在中等水平时生长量比发酵罐少得多。最大氧气摄取速率出现在对数生长期结束时的发酵罐中，此时生长量为最大值的 40%至 75%。在发酵罐中，亚硫酸盐氧化速率和氧气摄取速率与产生的总生长量都有合理的良好相关性。
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