DUGAN P R, LUNDGREN D G
J Bacteriol. 1965 Mar;89(3):825-34. doi: 10.1128/jb.89.3.825-834.1965.
Dugan, Patrick R. (Syracuse University, Syracuse, N.Y.), and Donald G. Lundgren. Energy supply for the chemoautotroph Ferrobacillus ferrooxidans. J. Bacteriol. 89:825-834. 1965.-A working model is proposed to explain dissimilatory ferrous iron oxidation by Ferrobacillus ferrooxidans, that is, oxidation linked to an energy source. The model is supported by experimental evidence reported here as well as in the literature. Polarographic assays of the culture medium demonstrated an iron "complex" involving oxygen. The initial "complex" would be oxygenated, but not oxidized because no electron transport has taken place. The "complex" is formed in solution or on the cell surface and is somehow reacted with iron oxidase (or oxygenase), resulting in the release of an electron. Either sulfate or a flavoprotein is suggested as involved in the initial electron-transfer link between iron and the cell. The electron is transported in the cell through a typical electron-transport system involving coenzyme Q(6), cytochrome c, and cytochrome a; oxygen is the final electron acceptor. Electron micrographs of intact and sectioned cells are included to show structural detail in support of the model.
杜根，帕特里克·R.（纽约州锡拉丘兹市雪城大学），以及唐纳德·G.伦德格伦。嗜铁氧化亚铁硫杆菌的能量供应。《细菌学杂志》89:825 - 834。1965年。——提出了一个工作模型来解释嗜铁氧化亚铁硫杆菌异化亚铁氧化作用，即与能量来源相关的氧化作用。该模型得到了本文以及文献中报道的实验证据的支持。对培养基进行极谱分析表明存在一种涉及氧气的铁“复合物”。最初的“复合物”会被氧化，但不会被氧化，因为尚未发生电子传递。“复合物”在溶液中或细胞表面形成，并以某种方式与铁氧化酶（或加氧酶）反应，导致电子释放。有人认为硫酸盐或黄素蛋白参与了铁与细胞之间的初始电子传递环节。电子在细胞内通过一个典型的电子传递系统进行传递，该系统涉及辅酶Q(6)、细胞色素c和细胞色素a；氧气是最终电子受体。文中还包括完整细胞和切片细胞的电子显微镜照片，以展示支持该模型的结构细节。
