Tuttle J H, Randles C I, Dugan P R
J Bacteriol. 1968 May;95(5):1495-503. doi: 10.1128/jb.95.5.1495-1503.1968.
Comparison of microbial content of acid-contaminated and nonacid-contaminated streams from the same geographical area indicated that nonacid streams contained relatively low numbers of acid-tolerant heterotrophic microorganisms. The acid-tolerant aerobes survived when acid entered the stream and actually increased in number to about 2 x 10(3) per ml until the pH approached 3.0. The organisms then represented the heterotrophic aerobic microflora of the streams comprised of a mixture of mine drainage and nonacid water. A stream which was entirely acid drainage did not have a similar microflora. Most gram-positive aerobic and anaerobic bacteria died out very rapidly in acidic water, and they comprised a very small percentage of the microbial population of the streams examined. Iron- and sulfur-oxidizing autotrophic bacteria were present wherever mine water entered a stream system. The sulfur-oxidizing bacteria predominated over iron oxidizers. Ecological data from the field were verified by laboratory experiments designed to simulate stream conditions.
对来自同一地理区域的酸性污染溪流和非酸性污染溪流中的微生物含量进行比较，结果表明，非酸性溪流中耐酸异养微生物的数量相对较少。当酸性物质进入溪流时，耐酸需氧菌能够存活下来，并且数量实际上会增加到每毫升约2×10³个，直到pH值接近3.0。此时，这些生物代表了由矿井排水和非酸性水混合而成的溪流中的异养需氧微生物群落。完全是酸性排水的溪流则没有类似的微生物群落。大多数革兰氏阳性需氧菌和厌氧菌在酸性水中很快死亡，在所检测的溪流微生物种群中所占比例非常小。只要矿井水进入溪流系统，就会有铁和硫氧化自养细菌存在。硫氧化细菌的数量超过铁氧化细菌。通过旨在模拟溪流条件的实验室实验，验证了现场的生态数据。
