Grillot-Courvalin C, Alter B J, Bach F H
J Immunol. 1977 Oct;119(4):1253-9.
The in vitro role of products of the H-2 complex in restimulation of secondary proliferative and cytotoxic responses has been studied. In this paper we have studied in particular the role of CD antigens, the target antigens for cytotoxic T lymphocytes. We have used UV-treated cells that express their CD antigens but no LD. Like others, we demonstrate that CD antigens alone are able to generate a secondary cytotoxic response and furthermore that these CD antigens must be the same as those used for primary sensitization. However, in contrast to the results of others, in some cases UV-treated cells were unable to restimulate such a response even when appropriate controls showed that the CD antigens on UV-treated cells were functional. Furthermore, in these cases the addition of an LD stimulus in the presence of UV-treated cells results in a secondary cytotoxic response that is significantly greater than that elicited by LD restimulation alone. Such potentiation is not observed if the CD antigens used for restimulation are different from those used for the primary sensitization. In addition, a non-H 2 differences(s), presumably the M1s locus, appears to be able to generate a secondary response. The implication of these results are discussed in terms of "memory" T lymphocytes in cell-mediated immunity.
已经研究了H-2复合体产物在再次刺激二次增殖和细胞毒性反应中的体外作用。在本文中，我们特别研究了细胞毒性T淋巴细胞的靶抗原CD抗原的作用。我们使用了经紫外线处理的细胞，这些细胞表达其CD抗原但不表达LD。与其他人一样，我们证明单独的CD抗原能够产生二次细胞毒性反应，而且这些CD抗原必须与用于初次致敏的抗原相同。然而，与其他人的结果相反，在某些情况下，即使适当的对照显示经紫外线处理的细胞上的CD抗原具有功能，经紫外线处理的细胞也无法再次刺激这种反应。此外，在这些情况下，在经紫外线处理的细胞存在下添加LD刺激会导致二次细胞毒性反应，该反应明显大于仅由LD再刺激引起的反应。如果用于再刺激的CD抗原与用于初次致敏的抗原不同，则不会观察到这种增强作用。此外，一种非H-2差异（可能是M1s位点）似乎能够产生二次反应。根据细胞介导免疫中的“记忆”T淋巴细胞对这些结果的意义进行了讨论。
