Alter B J, Grillot-Courvalin C, Bach M L, Zier K S, Sondel P M, Bach F H
J Exp Med. 1976 May 1;143(5):1005-14. doi: 10.1084/jem.143.5.1005.
Lymphocytes stimulated in mixed leukocyte cultures and left for 13-17 days, i.e. beyond their peak proliferative and cytotoxic reactivities, can be restimulated to give a secondary-type rapid and strong proliferative and cytotoxic response when confronted with cells of the original sensitizing cell donor. We have concerned ourselves primarily with the requirements of restimulation for the presence of LD and/or SD stimuli on the restimulating cells. (a) The low level cell-mediated lympholysis (CML) associated with LD differences in a primary CML can be restimulated to give a secondary-type response by those same LD antigens. (b) If the original sensitizing cells differ from the responding cells by both LD and SD antigens, restimulation with only the LD antigens, or third-party cells presumably carrying cross-reactive LD antigens, can restimulate the secondary CML responses directed against the SD antigens on the original sensitizing cells. (c) The presence of SD antigens on the restimulating cells that are cross-reactive with the primary sensitizing SD antigens (as determined in a primary CML) leads to the preferential activation of cytotoxic T lymphocytes reactive to those antigens although maximum cytotoxicity is still directed at cells carrying the original sensitizing SD antigens. A model to explain these results is presented.
在混合白细胞培养物中受到刺激并放置13 - 17天（即超过其增殖和细胞毒性反应峰值）的淋巴细胞，当与原始致敏细胞供体的细胞相遇时，可被再次刺激以产生二次型快速且强烈的增殖和细胞毒性反应。我们主要关注再次刺激时对再次刺激细胞上存在LD和/或SD刺激的要求。(a) 与初次细胞介导的淋巴细胞溶解（CML）中LD差异相关的低水平细胞介导的淋巴细胞溶解可被相同的LD抗原再次刺激以产生二次型反应。(b) 如果原始致敏细胞与反应细胞在LD和SD抗原方面均不同，仅用LD抗原或可能携带交叉反应性LD抗原的第三方细胞进行再次刺激，可再次刺激针对原始致敏细胞上SD抗原的二次CML反应。(c) 再次刺激细胞上存在与初次致敏SD抗原交叉反应的SD抗原（如在初次CML中所确定），尽管最大细胞毒性仍针对携带原始致敏SD抗原的细胞，但会导致对那些抗原具有反应性的细胞毒性T淋巴细胞优先活化。本文提出了一个解释这些结果的模型。
