广宿主范围质粒RK2的一个区域可使从苜蓿根瘤中分离出的苜蓿根瘤菌细胞中的质粒在植物体内稳定遗传。
A region of the broad-host-range plasmid RK2 causes stable in planta inheritance of plasmids in Rhizobium meliloti cells isolated from alfalfa root nodules.
作者信息
Weinstein M, Roberts R C, Helinski D R
机构信息
Department of Biology, University of California, San Diego, La Jolla 92093-0634.
出版信息
J Bacteriol. 1992 Nov;174(22):7486-9. doi: 10.1128/jb.174.22.7486-7489.1992.
Abstract
We demonstrate for the first time that the broad-host-range stabilization loci from plasmid RK2 cause total retention of plasmids in cells of Rhizobium meliloti during symbiosis with alfalfa. Two derivatives of plasmid RK2, pRK290 and a 7.3-kb mini-RK2 plasmid, were stabilized in R. meliloti cells isolated from root nodules by the insertion of a 3.2-kb DNA fragment or a smaller 0.8-kb DNA fragment derived from the RK2 stabilization region.
摘要
我们首次证明，来自质粒RK2的广宿主范围稳定基因座可使苜蓿根瘤菌在与苜蓿共生期间，其细胞中的质粒完全得以保留。通过插入源自RK2稳定区域的3.2 kb DNA片段或更小的0.8 kb DNA片段，质粒RK2的两个衍生物pRK290和一个7.3 kb的微型RK2质粒在从根瘤中分离出的苜蓿根瘤菌细胞中得到了稳定。
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A region of the broad-host-range plasmid RK2 causes stable in planta inheritance of plasmids in Rhizobium meliloti cells isolated from alfalfa root nodules.广宿主范围质粒RK2的一个区域可使从苜蓿根瘤中分离出的苜蓿根瘤菌细胞中的质粒在植物体内稳定遗传。
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