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禽类抗体。X. 一种针对2,4-二硝基苯基基团的抗体的纯化及特性

Fowl antibody. X. The purification and properties of an antibody to the 2,4-dinitrophenyl group.

作者信息

Orlans E

出版信息

Immunology. 1968 Jan;14(1):61-7.

PMID:4169726
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1409260/
Abstract

A preparation of fowl antibody to the 2,4-dinitrophenyl group (DNP), made from antisera to DNP-bovine γ-globulin by dissociation of a specific precipitate, was more than 90 per cent precipitable by DNP-ovalbumin. This antibody with an approximate molecular weight of 165,000 and the immunoelectrophoretic properties of γG-globulin, precipitated with antigen only in 8 per cent NaCl. It fixed fowl complement, but did not agglutinate tanned fowl red cells coated with DNP-protein. It had no skin sensitizing activity. Its amino acid composition was significantly different from that of mammalian antibodies. Some of the properties of another DNP-specific antibody that appeared later in immunization are also described.

摘要

一种由针对二硝基苯基（DNP）的鸡抗体制成的制剂，该抗体是通过解离特异性沉淀物从抗DNP - 牛γ球蛋白抗血清制备而来，它可被DNP - 卵清蛋白沉淀的比例超过90%。这种抗体的分子量约为165,000，具有γG - 球蛋白的免疫电泳特性，仅在8%氯化钠中与抗原沉淀。它能固定鸡补体，但不能凝集包被有DNP - 蛋白的鞣酸处理鸡红细胞。它没有皮肤致敏活性。其氨基酸组成与哺乳动物抗体有显著差异。文中还描述了免疫过程后期出现的另一种DNP特异性抗体的一些特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/1409260/40b8c61895c9/immunology00396-0073-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/1409260/9d92d90d9abe/immunology00396-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/1409260/c071ceb2135f/immunology00396-0074-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/1409260/19683c3c3a8b/immunology00396-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/1409260/40b8c61895c9/immunology00396-0073-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/1409260/9d92d90d9abe/immunology00396-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/1409260/c071ceb2135f/immunology00396-0074-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/1409260/19683c3c3a8b/immunology00396-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/165c/1409260/40b8c61895c9/immunology00396-0073-b.jpg

相似文献

1
Fowl antibody. X. The purification and properties of an antibody to the 2,4-dinitrophenyl group.禽类抗体。X. 一种针对2,4-二硝基苯基基团的抗体的纯化及特性
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引用本文的文献

1
Heterogeneity of the antigenic centres of albumin toward chicken antibodies.白蛋白抗原中心对鸡抗体的异质性。
Immunology. 1970 Jan;18(1):7-12.
2
Purification of chicken anti-bovine serum albumin antibody.鸡抗牛血清白蛋白抗体的纯化。
Immunology. 1971 Oct;21(4):637-48.
3
Passive cutaneous anaphylaxis in the chicken. Biological fractionation of the mediating antibody population.鸡的被动皮肤过敏反应。介导抗体群体的生物学分级分离。

本文引用的文献

1
IGG AND IGM ANTIBODIES IN FOWL SERUM.家禽血清中的免疫球蛋白G和免疫球蛋白M抗体。
Aust J Exp Biol Med Sci. 1965 Jul;43:451-4. doi: 10.1038/icb.1965.79.
2
FOWL ANTIBODY. VII. THE EFFECT OF NORMAL FOWL SERUM FACTORS ON SPECIFIC PRECIPITATION AND ON HAEMOLYSIS BY FOWL COMPLEMENT.禽抗体。VII. 正常禽血清因子对特异性沉淀反应及禽补体溶血作用的影响。
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PREPARATION OF PURIFIED ANTI-2,4-DINITROPHENYL ANTIBODIES.纯化抗2,4-二硝基苯基抗体的制备
Immunology. 1973 Jul;25(1):151-64.
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Immunology. 1972 Jan;22(1):165-75.
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Fowl antibody. IX. The different responses to the hapten and carrier moieties of 2,4-dinitrophenyl-bovine gamma-globulin.禽类抗体。IX. 对2,4 -二硝基苯基 - 牛γ球蛋白的半抗原和载体部分的不同反应。
Immunology. 1968 Jan;14(1):53-9.
Methods Med Res. 1964;10:94-102.
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ANTIBODY ACTIVITY IN DIFFERENT CHICKEN GLOBULINS.不同鸡球蛋白中的抗体活性。
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THE TEMPORAL SYNTHESIS OF CHICKEN ANTIBODIES. THE EFFECT OF SALT ON THE PRECIPITIN REACTION.鸡抗体的时间合成。盐对沉淀素反应的影响。
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6
Fowl antibody: IV. The estimation of haemolytic fowl complement.禽类抗体：IV. 溶血禽类补体的测定
Immunology. 1962 Nov;5(6):642-8.
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Molecular weight of rabbit gamma-globulin.
Nature. 1962 May 26;194:745-7. doi: 10.1038/194745a0.
8
Location of amino acid differences in the subunits of three rabbit antibodies.
Biochemistry. 1966 Feb;5(2):641-51. doi: 10.1021/bi00866a034.
9
Characteristics of the 19S and 7S response to bacteriophage 0X74 in the fowl.家禽对噬菌体0X74的19S和7S反应的特征
Immunology. 1966 Feb;10(2):169-78.
10
Properties of papain-digested chicken 7 S gamma-globulin.木瓜蛋白酶消化的鸡7Sγ球蛋白的特性
J Immunol. 1965 Nov;95(5):855-66.