Maxim P E, Veltri R W, Sprinkle P M, Pusateri R J
Trans Sect Otolaryngol Am Acad Ophthalmol Otolaryngol. 1977 Mar-Apr;84(2):234-8.
In characterizing the immunochemical and biochemical nature of middle ear effusions (MEE) of chronic serous otitis media (SOM), we have previously shown that MEE contain all major immunoglobulin classes, increased levels of several lysosomal enzymes, decreased total complement, but increased levels of C3. This report extends these observations by showing that MEE also contains C3 proactivator (C3PA) that can be activated by those organisms involved in acute otitis media, which confirms a functional alternate complement pathway. Furthermore, we have demonstrated the presence of soluble immune complexes in MEE using the Raji cell test. These data further support an immune complex mediated etiopathogenesis for chronic SOM.
在对慢性浆液性中耳炎（SOM）中耳积液（MEE）的免疫化学和生物化学性质进行表征时，我们之前已表明，MEE含有所有主要免疫球蛋白类别、几种溶酶体酶水平升高、总补体降低，但C3水平升高。本报告通过表明MEE还含有可被急性中耳炎相关病原体激活的C3前活化剂（C3PA）来扩展这些观察结果，这证实了一条功能性替代补体途径。此外，我们使用Raji细胞试验证明了MEE中存在可溶性免疫复合物。这些数据进一步支持慢性SOM的免疫复合物介导的发病机制。
