文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Mol Gen Genet. 1979 Jul 2;174(1):17-24. doi: 10.1007/BF00433300.

Total Neurospora crassa DNA was restricted with endonucleases and fragments carrying rRNA coding sequences were identified by hybridization with Xenopus laevis ribosomal DNA probes. 2. The repeating unit of the rRNA gene cluster was found to be 8.6 kbp, arranged in a head-to-tail fashion. 3. Digestion with Hind III yielded fragments of 3.4 kbp and 5.2 kbp and both were cloned. 4. Digestion with Eco RI yielded fragments of 2.2 kbp, 3.0 kbp and 3.4 kbp; the 3.0 kbp fragment was cloned. 5. Sequences coding for RNA (S-rRNA)1 of the smaller subribosomal particle were found (at least 90%) in the 2.2 kbp EcoRI subfragment of the 5.2 kbp Hind III fragment. 6. The coding sequences for the major RNA species (L-rRNA) of the larger subribosomal particle were located mainly (at least 95%) in the 3.4 kbp Hind III fragment. 7. For comparison, a Hind III digest of total yeast DNA was cloned and recombinants containing a 6.4 kbp rDNA fragment were isolated.

粗糙脉孢菌的总DNA用核酸内切酶进行酶切，携带rRNA编码序列的片段通过与非洲爪蟾核糖体DNA探针杂交来鉴定。2. 发现rRNA基因簇的重复单元为8.6千碱基对，呈头对头的方式排列。3. 用Hind III酶切产生3.4千碱基对和5.2千碱基对的片段，二者都被克隆。4. 用Eco RI酶切产生2.2千碱基对、3.0千碱基对和3.4千碱基对的片段；3.0千碱基对的片段被克隆。5. 在5.2千碱基对Hind III片段的2.2千碱基对EcoRI亚片段中发现了编码较小亚核糖体颗粒RNA（S-rRNA）1的序列（至少90%）。6. 较大亚核糖体颗粒主要RNA种类（L-rRNA）的编码序列主要（至少95%）位于3.4千碱基对的Hind III片段中。7. 作为比较，将总酵母DNA的Hind III酶切产物进行克隆，并分离出含有6.4千碱基对rDNA片段的重组体。

Mol Gen Genet. 1979 Jul 2;174(1):17-24. doi: 10.1007/BF00433300.

Total Neurospora crassa DNA was restricted with endonucleases and fragments carrying rRNA coding sequences were identified by hybridization with Xenopus laevis ribosomal DNA probes. 2. The repeating unit of the rRNA gene cluster was found to be 8.6 kbp, arranged in a head-to-tail fashion. 3. Digestion with Hind III yielded fragments of 3.4 kbp and 5.2 kbp and both were cloned. 4. Digestion with Eco RI yielded fragments of 2.2 kbp, 3.0 kbp and 3.4 kbp; the 3.0 kbp fragment was cloned. 5. Sequences coding for RNA (S-rRNA)1 of the smaller subribosomal particle were found (at least 90%) in the 2.2 kbp EcoRI subfragment of the 5.2 kbp Hind III fragment. 6. The coding sequences for the major RNA species (L-rRNA) of the larger subribosomal particle were located mainly (at least 95%) in the 3.4 kbp Hind III fragment. 7. For comparison, a Hind III digest of total yeast DNA was cloned and recombinants containing a 6.4 kbp rDNA fragment were isolated.

粗糙脉孢菌的总DNA用核酸内切酶进行酶切，携带rRNA编码序列的片段通过与非洲爪蟾核糖体DNA探针杂交来鉴定。2. 发现rRNA基因簇的重复单元为8.6千碱基对，呈头对头的方式排列。3. 用Hind III酶切产生3.4千碱基对和5.2千碱基对的片段，二者都被克隆。4. 用Eco RI酶切产生2.2千碱基对、3.0千碱基对和3.4千碱基对的片段；3.0千碱基对的片段被克隆。5. 在5.2千碱基对Hind III片段的2.2千碱基对EcoRI亚片段中发现了编码较小亚核糖体颗粒RNA（S-rRNA）1的序列（至少90%）。6. 较大亚核糖体颗粒主要RNA种类（L-rRNA）的编码序列主要（至少95%）位于3.4千碱基对的Hind III片段中。7. 作为比较，将总酵母DNA的Hind III酶切产物进行克隆，并分离出含有6.4千碱基对rDNA片段的重组体。