Nagelschmidt M, Fischer H, Engelhardt G H
Second Department of Surgery, University of Cologne, Germany.
J Surg Res. 1992 Nov;53(5):490-4. doi: 10.1016/0022-4804(92)90095-h.
Animal experiments have shown that administration of gelatin results in a deprivation of plasma fibronectin (FN) and impaired wound healing. For further elucidation of these findings a therapy study with purified human FN was performed in rats. Fifty animals received a standard burn injury of 1% body surface and were divided into five experimental groups. Positive controls given no further treatment or treated with solvent only served for estimation of normal healing. For a negative control, 10 animals received three intraperitoneal injections of gelatin (58 mg/kg body wt) on Days 0, 1, and 2 after injury. They exhibited a striking lack of plasma FN (Day 1) and a significant delay of wound contraction (Days 7 and 14). In the therapy groups each administration of gelatin was followed by an intraperitoneal or intracardiac injection of FN (58 mg/kg body wt) 1 hr later. In these animals the negative effect of gelatin upon plasma FN and wound contraction was prevented. According to this study wound healing is menaced by FN deficiency and can be optimized by substitution of exogenous FN.
动物实验表明，给予明胶会导致血浆纤连蛋白（FN）缺乏，伤口愈合受损。为进一步阐明这些发现，在大鼠中进行了一项使用纯化人FN的治疗研究。50只动物遭受了1%体表面积的标准烧伤，并被分为五个实验组。未接受进一步治疗或仅用溶剂治疗的阳性对照组用于评估正常愈合情况。作为阴性对照，10只动物在受伤后第0、1和2天接受了三次腹腔注射明胶（58毫克/千克体重）。它们在第1天表现出明显的血浆FN缺乏，伤口收缩在第7天和第14天显著延迟。在治疗组中，每次给予明胶1小时后，腹腔内或心内注射FN（58毫克/千克体重）。在这些动物中，明胶对血浆FN和伤口收缩的负面影响得到了预防。根据这项研究，伤口愈合受到FN缺乏的威胁，通过外源性FN替代可以使其得到优化。
