Nakada M, Nakada K, Kitagawa H, Kawaguchi F, Hoson M, Wakisaka M, Kashimura T, Ohkawa I, Sato Y, Furuta T
Division of Pediatric Surgery, St Marianna University School of Medicine, Kawasaki, Japan.
J Pediatr Surg. 1998 Nov;33(11):1699-702. doi: 10.1016/s0022-3468(98)90613-3.
BACKGROUND/PURPOSE: Fibronectin (FN) plays an important role in fibrin matrix formation during the wound healing process. The authors investigated whether exogenous FN increases the bursting strength (BS) of surgical wounds in malnourished rats.
Ninety rats were grouped according to three nutritional conditions (n = 30 in each group). All animals underwent a transverse celiotomy after 3 weeks of feeding, and FN (2 mg/body/day) was given postoperatively to 15 rats in each of the three groups. Wound BS was measured on postoperative days (PODs) 3, 5, and 7 after removal of the sutures.
BS in normally nourished rats (group N; 142.6+/-23.4 mmHg) was significantly higher than that in protein malnourished rats (group PM, 110.2+/-11.2 mm Hg) and protein/ calorie (Cal)-malnourished rats (group PCM, 76.5+/-10.7 mm Hg) on POD 7 (P<.01). However, BS values for groups PM + FN (147.0+/-21.1 mmHg) and PCM + FN (115.1+/-28.9 mm Hg) were intensified significantly in comparison with groups PM and PCM (P<.01). Plasma FN levels in rats of the three FN nontreated groups were similarly decreased on POD 3 or 5, but returned to the preoperative level on POD 7, whereas those for the other three FN-treated groups increased after POD 3.
Intravenous administration of FN might strengthen the weakened wounds of malnourished animals.
背景/目的：纤连蛋白（FN）在伤口愈合过程中纤维蛋白基质形成中起重要作用。作者研究了外源性FN是否能增加营养不良大鼠手术伤口的抗破裂强度（BS）。
90只大鼠根据三种营养状况分组（每组n = 30）。所有动物在喂养3周后接受横向剖腹术，三组中每组15只大鼠术后给予FN（2mg/体/天）。在拆除缝线后的术后第3、5和7天测量伤口BS。
在术后第7天，正常营养大鼠组（N组；142.6±23.4mmHg）的BS显著高于蛋白质营养不良大鼠组（PM组，110.2±11.2mmHg）和蛋白质/热量（Cal）营养不良大鼠组（PCM组，76.5±10.7mmHg）（P<0.01）。然而，与PM组和PCM组相比，PM + FN组（147.0±21.1mmHg）和PCM + FN组（115.1±28.9mmHg）的BS值显著增强（P<0.01）。三个未用FN处理组的大鼠血浆FN水平在术后第3天或第5天同样降低，但在术后第7天恢复到术前水平，而其他三个用FN处理组的血浆FN水平在术后第3天后升高。
静脉注射FN可能会增强营养不良动物减弱的伤口。
