金属硫蛋白免疫反应性在肌萎缩侧索硬化症患者的脊髓中增强。

Metallothionein immunoreactivity is increased in the spinal cord of patients with amyotrophic lateral sclerosis.

作者信息

Sillevis Smitt P A, Blaauwgeers H G, Troost D, de Jong J M

机构信息

Department of Neurology, Academic Medical Center, Amsterdam, Netherlands.

出版信息

Neurosci Lett. 1992 Sep 14;144(1-2):107-10. doi: 10.1016/0304-3940(92)90727-o.

DOI:10.1016/0304-3940(92)90727-o

PMID:1436688

Abstract

Sections of the spinal cord from 10 patients with classic amyotrophic lateral sclerosis and from 10 control cases were examined by immunocytochemical methods to localize metallothionein. Metallothionein immunoreactivity was seen in the nucleus and cytoplasm of a subset of astrocytes, largely confined to the gray matter. Also, diffuse gray matter staining was observed, probably representing small glial fibers. Astrocytic metallothionein immunoreactivity (P less than 0.01) and strong gray matter matrix staining (P less than 0.03) was increased in the spinal cords from patients with amyotrophic lateral sclerosis. Although compatible with induction by metals, increased metallothionein expression in the spinal cords from patients with amyotrophic lateral sclerosis may also have resulted from inflammation or gliosis.

摘要

采用免疫细胞化学方法对10例经典肌萎缩侧索硬化患者和10例对照者的脊髓切片进行检查，以定位金属硫蛋白。在一部分星形胶质细胞的细胞核和细胞质中可见金属硫蛋白免疫反应性，主要局限于灰质。此外，还观察到弥漫性灰质染色，可能代表小胶质纤维。肌萎缩侧索硬化患者脊髓中星形胶质细胞金属硫蛋白免疫反应性（P<0.01）和强烈的灰质基质染色（P<0.03）增加。虽然与金属诱导相符，但肌萎缩侧索硬化患者脊髓中金属硫蛋白表达增加也可能是由炎症或胶质增生引起的。