FREUND J, THOMPSON G E, LIPTON M M
J Exp Med. 1955 Jun 1;101(6):591-604. doi: 10.1084/jem.101.6.591.
Guinea pig testicles were extracted with acetic acid; the extract was purified by removing material in consecutive precipitations with 30 per cent saturated ammonium-sulfate, trichloracetic acid, and chloroform. The solution so purified, when administered with complete adjuvants, was highly active in inducing impairment of spermatogenesis in guinea pigs. The activity resisted autoclaving at 15 pounds' pressure for 20 minutes, proteolytic enzymes, and formamide. Anaphylactic shock and cutaneous reaction to the purified homologous extract occurred in guinea pigs sensitized by the extract combined with adjuvants. For the production of aspermatogenesis it was essential to incorporate killed mycobacteria into the water-in-oil emulsion containing the antigen; but anaphylactic sensitization did not require the presence of mycobacteria.
用醋酸提取豚鼠睾丸；提取物通过用30%饱和硫酸铵、三氯乙酸和氯仿连续沉淀去除物质来纯化。如此纯化的溶液与完全佐剂一起给药时，在诱导豚鼠精子发生损伤方面具有高度活性。该活性能抵抗15磅压力下20分钟的高压灭菌、蛋白水解酶和甲酰胺。对经提取物与佐剂致敏的豚鼠，纯化的同源提取物会引发过敏休克和皮肤反应。为了产生无精子症，将灭活的分枝杆菌加入含抗原的油包水乳剂中是必不可少的；但过敏致敏并不需要分枝杆菌的存在。
