Kay D J, Boettcher B
Department of Biological Sciences, University of Newcastle, Callaghan, NSW, Australia.
Reprod Fertil Dev. 1992;4(2):175-81. doi: 10.1071/rd9920175.
To eliminate the possibility of immunological infertility in spontaneously infertile and re-anastomosed men, a screening test that can be applied directly to semen is desirable. The SpermMar test is one such possibility. In this study, indirect tests for sperm antibodies using the commercial SpermMar test have been applied to a panel of sera whose reactions in the tube slide agglutination test (TSAT), gelatin agglutination test (GAT) and sperm immobilization test (SIT) for sperm antibodies are well characterized. The results from the SpermMar tests are compared directly with those obtained from Immunobead tests carried out at the same time. Results from screening tests performed on 30 sera confirmed complete correspondence between the GAT, SpermMar and Immunobead tests. When sera were titrated, the Immunobead test proved slightly more sensitive than the GAT and the SpermMar test was slightly more sensitive than the Immunobead test. The SpermMar test proved easier to use and to assess than the Immunobead test and it is recommended for consideration as a screening procedure for sperm antibodies despite the fact that at this stage only IgG antibodies can be detected.
为排除自然不育及输精管再吻合术后男性免疫性不育的可能性，需要一种可直接应用于精液的筛查试验。精子膜抗原检测（SpermMar试验）就是这样一种可能的方法。在本研究中，使用商业化的SpermMar试验对精子抗体进行间接检测，并应用于一组血清，这些血清在精子抗体的试管玻片凝集试验（TSAT）、明胶凝集试验（GAT）和精子制动试验（SIT）中的反应已得到充分表征。将SpermMar试验的结果直接与同时进行的免疫珠试验结果进行比较。对30份血清进行筛查试验的结果证实，GAT、SpermMar试验和免疫珠试验结果完全一致。当对血清进行滴定检测时，免疫珠试验比GAT稍敏感，而SpermMar试验比免疫珠试验稍敏感。结果表明，SpermMar试验比免疫珠试验更易于使用和评估，尽管目前该试验仅能检测IgG抗体，但仍建议将其作为精子抗体的筛查方法加以考虑。
