Khole V, Hegde U
Institute for Research in Reproduction, Parel, Bombay, India.
Reprod Fertil Dev. 1992;4(2):223-30. doi: 10.1071/rd9920223.
An auto-anti-idiotypic approach was used to generate mouse monoclonal anti-idiotypic antibody E9F11F6 (Ab2) to progesterone. A conjugate of 4-pregnane 3,20 dione and bovine serum albumin was used as the immunogen. E9F11F6 bound to a rabbit anti-progesterone antibody in a linear concentration-dependent manner. It inhibited the binding of [3H]progesterone to a monoclonal anti-progesterone antibody, E9D5 (Ab1); this inhibition was concentration dependent. Results from chase experiments showed that this inhibitory activity of Ab2 was not due to steric hindrance but was a result of direct binding to the ligand combining site. Indirect immunofluorescence studies also showed that Ab2 and progesterone bound to similar binding sites on Ab1. Overall, the results suggest that E9F11F6 contains an internal image of at least part of the progesterone molecule, and that it can be classified as an Ab2 beta antibody. This anti-idiotypic antibody may be of value in further studies of endocrine and reproductive physiology.
采用自身抗独特型方法制备了针对孕酮的小鼠单克隆抗独特型抗体E9F11F6（Ab2）。以4-孕烷-3,20-二酮与牛血清白蛋白的偶联物作为免疫原。E9F11F6以线性浓度依赖方式与兔抗孕酮抗体结合。它抑制了[3H]孕酮与单克隆抗孕酮抗体E9D5（Ab1）的结合；这种抑制作用是浓度依赖性的。追踪实验结果表明，Ab2的这种抑制活性并非由于空间位阻，而是直接结合到配体结合位点的结果。间接免疫荧光研究还表明，Ab2和孕酮结合到Ab1上的相似结合位点。总体而言，结果表明E9F11F6包含至少部分孕酮分子的内影像，并且它可被归类为Ab2β抗体。这种抗独特型抗体可能在进一步研究内分泌和生殖生理学方面具有价值。
