Muraoka T, Okuda H
J Biochem. 1977 Aug;82(2):529-33.
A novel method for the estimation of fibrinolytic activity is proposed. In this method, a fibrin clot suspension is used as a substrate (fibrin is known to be a physiological substrate of plasmin). The fibrin clot suspension was prepared by homogenization of human fibrin clots. With this method, we found that free fatty acids inhibited the plasmin activity, and long-chain, unsaturated free fatty acids had a particularly strong inhibitory action on plasmin. As regards the mechanism of the inhibitory action, free fatty acids may not inhibit complex formation between plasmin and fibirin, but may make it impossible for plasmin to act on fibrin due to deformation of the surface of the fibrin clot.
提出了一种估算纤溶活性的新方法。在该方法中，使用纤维蛋白凝块悬液作为底物（已知纤维蛋白是纤溶酶的生理底物）。纤维蛋白凝块悬液通过人纤维蛋白凝块的匀浆制备。用这种方法，我们发现游离脂肪酸抑制纤溶酶活性，长链不饱和游离脂肪酸对纤溶酶具有特别强的抑制作用。关于抑制作用的机制，游离脂肪酸可能不会抑制纤溶酶与纤维蛋白之间的复合物形成，但可能由于纤维蛋白凝块表面的变形而使纤溶酶无法作用于纤维蛋白。
