Yamashita U, Shevach E M
J Immunol. 1977 Nov;119(5):1584-8.
Only 15 to 25% of purified oil-induced guinea pig macrophages could be lysed by treatment with anti-Ia serum and C. Those cells remaining alive after treatment were not damaged and were metabolically active since they readily phagocytized latex beads. However, the "Ia-negative" macrophages were markedly deficient in their ability to present protein antigens to immune T lymphocytes and to function as stimulator cells when mixed with allogeneic T cells in the mixed leukocyte reaction. It thus appears that Ia antigens are expressed on a subpopulation of macrophages and that this subpopulation plays a critical role in the activation of T cell proliferation to soluble protein antigens and to alloantigens.
仅15%至25%经纯化油诱导的豚鼠巨噬细胞在用抗Ia血清和补体处理后可被裂解。处理后仍存活的细胞未受损伤且代谢活跃，因为它们能轻易吞噬乳胶微珠。然而，“Ia阴性”巨噬细胞在将蛋白质抗原呈递给免疫T淋巴细胞以及在混合淋巴细胞反应中与同种异体T细胞混合时作为刺激细胞发挥功能的能力上明显不足。因此，似乎Ia抗原在巨噬细胞的一个亚群上表达，并且该亚群在T细胞对可溶性蛋白质抗原和同种异体抗原的增殖激活中起关键作用。
