Carrie S, Hutton D A, Birchall J P, Green G G, Pearson J P
Department of Physiological Sciences, University of Newcastle upon Tyne, U.K.
Acta Otolaryngol. 1992;112(3):504-11. doi: 10.3109/00016489209137432.
Middle ear effusions from children undergoing myringotomy were classified into thick (mucoid) and thin (serous) on the basis of their flow properties. Their composition was analysed and their rheological properties measured. The viscosity of the effusions was measured using a Contraves low shear viscometer and expressed as specific viscosity per mg/ml of non-dialysable solids present. In order to measure the effusion viscosity it was necessary to solubilize the effusion by mild homogenisation in a phosphate buffer pH 6.7 containing a cocktail of proteolytic inhibitors. The viscosity of mucoid effusions was significantly greater than that of the serous effusions. There was a small but measurable amount of proteolytic activity in the effusions, range 0.05-1.79 micrograms/mg of non-dialysable solids. This proteolytic activity was not significantly different between the thick and thin effusions and was therefore unlikely to explain the difference in viscosity. Analysis of the constituents of the effusions showed that glycoprotein and DNA but not protein nor lipid were significantly higher in the mucoid effusions compared to the serous effusions. The viscosity of the effusions correlated with the glycoprotein concentration but not with the protein or lipid concentration. Under certain circumstances the DNA concentration did correlate with the viscosity of the effusion. However, digestion with a proteinase free DNase did not reduce the viscosity of the effusion. These results demonstrate that classifying effusions as thick and thin based on visual inspection and flow properties is valid and that the only constituent present in the effusions that determines viscosity is mucin.
接受鼓膜切开术的儿童中耳积液，根据其流动特性分为浓稠（黏液样）和稀薄（浆液样）两类。对其成分进行了分析，并测量了流变学特性。积液的黏度使用Contraves低剪切黏度计进行测量，并表示为每毫克/毫升不可透析固体的比黏度。为了测量积液黏度，有必要通过在含有蛋白水解抑制剂混合物的pH 6.7磷酸盐缓冲液中进行温和匀浆来使积液溶解。黏液样积液的黏度显著高于浆液样积液。积液中存在少量但可测量的蛋白水解活性，范围为0.05 - 1.79微克/毫克不可透析固体。这种蛋白水解活性在浓稠和稀薄积液之间没有显著差异，因此不太可能解释黏度差异。对积液成分的分析表明，与浆液样积液相比，黏液样积液中的糖蛋白和DNA含量显著更高，而蛋白质和脂质含量则不然。积液的黏度与糖蛋白浓度相关，但与蛋白质或脂质浓度无关。在某些情况下，DNA浓度确实与积液黏度相关。然而，用无蛋白酶的DNA酶消化并没有降低积液的黏度。这些结果表明，基于目视检查和流动特性将积液分为浓稠和稀薄是有效的，并且积液中决定黏度的唯一成分是黏蛋白。
