Johnson I J, Brooks T, Hutton D A, Birchall J P, Pearson J P
Department of Otorhinolaryngology, Queens Medical Centre, University Hospital, Nottingham, U.K.
Laryngoscope. 1997 May;107(5):684-9. doi: 10.1097/00005537-199705000-00024.
The aim of this study was to clarify the site of primary pathology in otitis media with effusion. Effusions were collected from 64 children with bilateral effusions at the time of myringotomy. The rheological properties and biochemical compositions of effusions were measured for 23 pairs of effusions, and the levels of the inflammatory mediators TNF alpha, IL-1beta, and IL-8 were measured in 41 pairs using specific enzyme-linked immunosorbent assays (ELISAs). Measurements from paired ears were compared using analysis of variance (ANOVA) tests and significant differences were found for reduced specific viscosity, mucin content, protein content, and levels of IL-8. The results demonstrate that the two ears have different immunological processes or rates of processes which might explain the significantly different rheological properties of effusions. This suggests that each ear undergoes pathological changes independently and has implications for using the opposite ear as a control in clinical trials.
本研究的目的是明确分泌性中耳炎的主要病理部位。在鼓膜切开术时，从64例双侧积液的儿童中收集积液。对23对积液进行了流变学特性和生化成分测定，并用特异性酶联免疫吸附测定法（ELISA）对41对积液中的炎症介质肿瘤坏死因子α、白细胞介素-1β和白细胞介素-8水平进行了测定。采用方差分析（ANOVA）检验对双耳的测量结果进行比较，发现比浓粘度降低、粘蛋白含量、蛋白质含量和白细胞介素-8水平存在显著差异。结果表明，双耳具有不同的免疫过程或进程速率，这可能解释了积液流变学特性的显著差异。这表明每只耳朵独立发生病理变化，这对在临床试验中使用对侧耳朵作为对照具有启示意义。
